RIPA Lysis Buffer System A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included.

RIPA Lysis Buffer System

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RIPA Lysis Buffer System is rated 4.71 out of 5 by 35.
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Noms alternatifs: RIPA Lysis Buffer System also known as RIPA (Radioimmunoprecipitation) Lysis Buffer System
Application: RIPA Lysis Buffer System® is a high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
* Consulter le Certificat d'Analyses pour les données spécifiques à un lot (incluant la teneur en eau).
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RIPA (Radioimmunoprecipitation) Lysis Buffer System is used to lyse cells and tissue, for radio immunoprecipitation assay (RIPA). RIPA lysis buffer has stronger denaturing capabilities than NP-40 (sc-281108) or Triton X-100 (sc-29112) and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts. RIPA Lysis Buffer System includes protease inhibitors, making it ready for use in mammalian cell and tissue lysis.

sc-24948, 50 mL - Components supplied in four vials:
VIAL 1: 50 mL 1X lysis buffer (pH 7.4 ±0.1)
VIAL 2: 500 μL (200mM) PMSF in DMSO
VIAL 3: 500 μL protease inhibitor cocktail in DMSO
VIAL 4: 500 μL (100mM) sodium orthovanadate in water

sc-24948A, 500 mL - Components supplied in four vials:
VIAL 1: 500 mL 1X lysis buffer
VIAL 2: 5 ml (200mM) PMSF in DMSO
VIAL 3: 5 ml protease inhibitor cocktail in DMSO
VIAL 4: 5 ml (100mM) sodium orthovanadate in water


Références

1. Poirier, F., et al. 1982. Int. J. Cancer. 29: 69-76. PMID: 6277805

2. DeSeau, V., et al. 1987. J. Cell. Biochem. 35: 113-128. PMID: 2448318

3. Bolen, J.B., et al. 1987. Oncogene Res. 1: 149-168. PMID: 2453014

4. Ngoka, L.C. 2008. Proteome Sci. 6: 30. PMID: 18950484

Utilisation :
1. Immediately prior to lysing cells, combine 10 μl PMSF solution, 10 μl sodium orthovanadate solution and 10-20 μl protease inhibitor cocktail solution per ml of 1X RIPA Lysis buffer to prepare complete RIPA.

2. Use 3 ml complete RIPA per gram of tissue

- OR 1 ml complete RIPA per 2.0x107 cells in suspension

- OR 0.6 ml complete RIPA per subconfluent monolayer on a 100 mm plate.
État Physique :
Liquid
STOCKAGE :
Store at -20° C
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.

Télécharger la Fiche de Sécurité/ SDS (MSDS)

CERTIFICAT D'ANALYSE

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RIPA Lysis Buffer System  Références bibliographiques

Consultez les publications faisant référence à l' utilisation de RIPA Lysis Buffer System. Cliquez sur le lien pour afficher lentrée PubMed .

Citations 1 à 10 sur un total de 3873

PMID: # 33532035  Liu, S.|Flores, JJ.|Li, B.|Deng, S.|Zuo, G.|Peng, J.|Tang, J.|Zhang, JH.| et al. 2021. Oxid Med Cell Longev. 2021: 5913424.

PMID: # 33821473  Roshani Asl, E.|Rasmi, Y.|Baradaran, B.| et al. 2021. J Cell Physiol.

PMID: # 34136360  Kobara, M.|Nessa, N.|Toba, H.|Nakata, T.| et al. 2021. Toxicol Rep. 8: 1087-1097.

PMID: # 33505114  Sun, Z.|Qiu, Z.|Ma, B.|Wang, Z.| et al. 2021. Cytotechnology. 73: 63-70.

PMID: # 33886620  Kim, SE.|Kim, J.|Lee, JY.|Lee, SB.|Paik, JS.|Yang, SW.| et al. 2021. PLoS One. 16: e0249988.

PMID: # 33546185  Al-Beltagi, S.|Preda, CA.|Goulding, LV.|James, J.|Pu, J.|Skinner, P.|Jiang, Z.|Wang, BL.|Yang, J.|Banyard, AC.|Mellits, KH.|Gershkovich, P.|Hayes, CJ.|Nguyen-Van-Tam, J.|Brown, IH.|Liu, J.|Chang, KC.| et al. 2021. Viruses. 13:

PMID: # 33592546  Asadzadeh, Z.|Mansoori, B.|Mohammadi, A.|Kazemi, T.|Mokhtarzadeh, A.|Shanehbandi, D.|Hemmat, N.|Derakhshani, A.|Brunetti, O.|Safaei, S.|Aghajani, M.|Najafi, S.|Silvestris, N.|Baradaran, B.| et al. 2021. Biomed Pharmacother. 137: 111364.

PMID: # 33566225  Shateri, H.|Manafi, B.|Tayebinia, H.|Karimi, J.|Khodadadi, I.| et al. 2021. Mol Biol Rep. 48: 1181-1191.

PMID: # 34251942  Cruz-López, O.|Ner, M.|Nerín-Fonz, F.|Jiménez-Martínez, Y.|Araripe, D.|Marchal, JA.|Boulaiz, H.|Gutiérrez-de-Terán, H.|Campos, JM.|Conejo-García, A.| et al. 2021. J Enzyme Inhib Med Chem. 36: 1553-1563.

PMID: # 33977960  Savcı, Y.|Kırbaş, OK.|Bozkurt, BT.|Abdik, EA.|Taşlı, PN.|Şahin, F.|Abdik, H.| et al. 2021. Food Funct. 12: 5144-5156.

Citations 1 à 10 sur un total de 3873
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