Potassium Phosphate Monobasic solution CAS: 7778-77-0
MF: KH2PO4
A convenient 0.2 µm filtered, 1M stock solution for making phosphate buffers.

Potassium Phosphate Monobasic solution (CAS 7778-77-0)

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Application: Potassium Phosphate Monobasic solution is a convenient 0.2 µm filtered, 1M stock solution for making phosphate buffers
Numéro CAS: 7778-77-0
Formule Moléculaire: KH2PO4
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
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Potassium Phosphate Monobasic solution is a convenient stock solution for making phosphate buffers. Widely used in molecular biology, biochemistry, and chromatography. Potassium phosphate occurs in several forms: monobasic (KH2PO4), dibasic (K2HPO4), and tribasic (K3PO4). Neutral potassium phosphate buffer solutions may be prepared with a mixture of the monobasic and dibasic forms to varying degrees, depending on the desired pH. Potassium phosphate buffers are very useful in numerous applications, but with the following limitations; precipitation of Ca2+ and Mg2+, inhibition of restriction enzyme activity, and interference in DNA ligation or bacterial transformation protocols. Has been used to study the effects of freezing and thawing on the stability of proteins sensitive to conformational changes; it was found that potassium phosphate buffers offered improved pH stability as opposed to sodium phosphate buffers. Potassium phosphate has also been used for the extraction of keratohyalin protein from bovine tissue.
Additional forms available:
Potassium Phosphate, Dibasic, Anhydrous (sc-203210)
Potassium Phosphate, Monobasic (sc-203211)
Potassium phosphate tribasic (sc-250766)
Potassium phosphate tribasic monohydrate (sc-250767)
Potassium phosphate dibasic solution (sc-301600)


Références

1. Ugel, A.R. and Idler, W. 1972. J. Cell Biol. 52: 453-464. PMID: 5057980
2. Pikal-Cleland, K.A., et al. 2000. Arch. Biochem. Biophys. 384: 398-406. PMID: 11368330
3. Sambrook, et al. 1989. Rnai: Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press. New York. Cold Spring Harbor. Vol. 3.
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Utilisation :
Potassium Phosphate Monobasic solution is prepared with high-quality reagents and 0.2 μm filtered; however it has not been evaluated for use in cell culture. Please employ appropriate sterilization techniques for the intended application.
Formulation :
1.0 M in solution
État Physique :
Liquid
Solubilité :
Soluble in water.
STOCKAGE :
Store at room temperature
Densité :
0.10 g/mL
Valeurs pK :
pKa: 1.97 (Predicted)
Pour la Recherche Uniquement. Non conforme pour le Diagnostic ou pour une Utilisation Thérapeutique.
WGK Allemagne :
1
Index Merck :
14: 7659
Numéro MDL :
MFCD00011401
Numéro CE :
231-791-2

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