Anticorps MCP-1 (ECE.2) recommandé pour la détection de murine MCP-1 d'origine mouse par WB, IP et IF

Anticorps MCP-1 (ECE.2): sc-52701

Anticorps MCP-1 (ECE.2) est évalué 3.9 de 5 de 9.
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Fiches techniques

    • L'anticorps MCP-1 ECE.2 est un monoclonal IgG1 de rat, cité dans 24 publications, fourni en 100 µg/ml
    • dirigé contre MCP-1 d'origine mouse
    • recommandé pour la détection de murine MCP-1 d'origine mouse par WB, IP et IF
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de MCP-1 (ECE.2): sc-52701.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for MCP-1 Antibody (ECE.2). This work is in progress.
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Dear Sir/Madam, Which secondary antibody can I use? Because I didn't find secondary antibody for rat IgG1. In your company just this antibody can use for checking mouse MCP-1. Thank you very much!

Posée par: ruozhi
Thank you for your question. MCP-1 Antibody (ECE.2) is a rat monoclonal IgG1. We recommend using any secondary antibody that is anti-rat IgG. Unfortunately, we do not currently offer any secondary antibodies anti-rat IgG at this time. Please check back for updates in the future!
Répondue par: Technical Support
Date de publication: 2019-08-17

I am using MCP-1 (ECE.2): sc-52701 for immunofluorescence, what dilution of this antibody should I use?

Posée par: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 5 de 5 de par the antibody works great! I bought it for our mice aorta tissue staining, it works very well.
Date de publication: 2019-02-05
Rated 3 de 5 de par WB 2C12 secreted medium It doesn't work in the conditioned medium of C2C12 cells in Westren blotting at the diluition of 1:1000
Date de publication: 2018-10-05
Rated 4 de 5 de par Good antibody This antibody worked very well with fixed frozen mouse tissue sections for IF
Date de publication: 2018-10-03
Rated 4 de 5 de par 一般吧 it is a good antibody suitable for westernblot 一般般 这个不好孵
Date de publication: 2017-08-30
Rated 5 de 5 de par good antibody Very small MW only 12kda, I recommend use 0.22um PVDF and expose long time and loading more than 60ug protein,1:200 antibody been diluted.
Date de publication: 2017-07-01
Rated 5 de 5 de par specific target band The specificity of MCP-1 antibody is very good,i have a good specific band use dilution 1:500.
Date de publication: 2017-05-27
Rated 5 de 5 de par clear band seen We observed clear band when diluted 1:500 ,Great help to us, strongly recommended for mouse brain tissue detection..
Date de publication: 2017-05-23
Rated 2 de 5 de par no bands in THP-1 WB signal can not be obtained using this antibody sample at 1:500 dilultion against THP-1 whole cell lysates. Good and quick service. Thanks.
Date de publication: 2017-05-19
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