Anticorps MCP-1-4/eotaxin (B-2) recommandé pour la détection de MCP-1, MCP-2, MCP-3, MCP-4 and eotaxin d'origine human par WB, IP, IF, IHC(P) et ELISA

Anticorps MCP-1-4/eotaxin (B-2): sc-377082

Anticorps MCP-1-4/eotaxin (B-2) est évalué 4.0 de 5 de 6.
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Fiches techniques

    • Anti-L'anticorps MCP-1-4/eotaxin B-2 est un monoclonal IgG1 κ L'Anticorps MCP-1-4/eotaxin, cité dans 3 publications, fourni en 200 µg/ml
    • raised against amino acids 1-99 representing full length MCP-1 of human origin
    • L'Anticorps MCP-1-4/eotaxin (B-2) est recommandé pour la détection de MCP-1, MCP-2, MCP-3, MCP-4 and eotaxin d'origine human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps MCP-1-4/eotaxin (B-2) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de MCP-1-4/eotaxin (B-2): sc-377082.
    • m-IgG1 BP-HRP et m-IgGκ BP-HRP sont les réactifs secondaires recommandés pour la détection de l'anticorps MCP-1-4/eotaxin (B-2) pour des applications de WB and IHC(P). Ces réactifs sont maintenant proposés en kit avec l'anticorps MCP-1-4/eotaxin (B-2) (voir Informations pour la commande ci-dessous).
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Can MCP-1-4/eotaxin (B-2): sc-377082 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Posée par: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MCP-1-4/eotaxin (B-2): sc-377082 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Répondue par: Technical Support
Date de publication: 2017-02-24
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Rated 3 de 5 de par okay antibody I tried this antibody in the ratio 1:1000 to detect CCL2 in NHBE whole cell lysate and luckily we got a band around 12kDa for the first time. But after that we tried in Beas2b cell lysate and again in NHBE lysate too but we couldn't see the band. Maybe it will work only in freshly prepared antibody so its not a user-friendly.
Date de publication: 2017-03-14
Rated 5 de 5 de par only for WB we do human samples stimulated by IL1B, and we see spicific band at 11kD, and also there is a none spicific band at 40kD. But we do not consider it could do IHC-P.
Date de publication: 2016-12-14
Rated 2 de 5 de par Robust band seen in WB using human lung tissue Robust band seen in WB using human lung tissue extract. -SCBT QC
Date de publication: 2015-04-20
Rated 4 de 5 de par Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date de publication: 2015-02-03
Rated 5 de 5 de par Published WB and IHC data with mouse samples Published WB and IHC data with mouse samples - SCBT Publication Review
Date de publication: 2015-01-19
Rated 5 de 5 de par Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date de publication: 2013-01-18
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