MAP-4 Antibody (F-3): sc-365187

MAP-4 Antibody (F-3) is a mouse monoclonal IgG1 antibody that detects MAP-4 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). MAP-4 is a crucial microtubule-associated protein that plays a significant role in the assembly and stability of microtubules, which are essential components of the cytoskeletal network. MAP-4 is primarily expressed in vital organs such as the kidney, lung, liver, testis, and spleen, where MAP-4 contributes to the organization and dynamics of the microtubule system. Notably, MAP-4 contains three 18-amino acid repeats that are homologous to those found in other microtubule-associated proteins, highlighting MAP-4′s structural importance in facilitating microtubule assembly and influencing the spatial distribution of microtubules within cells. Proper functioning of MAP-4 is critical for various cellular processes, including the formation of the mitotic spindle during cell division, which ensures accurate chromosome segregation. Disruptions in MAP-4 function can lead to cellular instability and have been implicated in various diseases, making MAP-4 a vital target for research in cell biology and pathology. Anti-MAP-4 antibody (F-3) is an invaluable tool for researchers aiming to explore the intricate roles of microtubule dynamics in health and disease.

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MAP-4 Antibody (F-3) References:

1. Microtubule-associated protein 4 (MAP4) regulates assembly, protomer-polymer partitioning and synthesis of tubulin in cultured cells.  |  Nguyen, HL., et al. 1999. J Cell Sci. 112 (Pt 12): 1813-24. PMID: 10341201
2. Truncation of the projection domain of MAP4 (microtubule-associated protein 4) leads to attenuation of microtubule dynamic instability.  |  Permana, S., et al. 2005. Cell Struct Funct. 29: 147-57. PMID: 15840946
3. An isoform of microtubule-associated protein 4 inhibits kinesin-driven microtubule gliding.  |  Tokuraku, K., et al. 2007. J Biochem. 141: 585-91. PMID: 17317690
4. Distinct neuronal localization of microtubule-associated protein 4 in the mammalian brain.  |  Tokuraku, K., et al. 2010. Neurosci Lett. 484: 143-7. PMID: 20727942
5. The p38/mitogen-activated protein kinase pathway is implicated in lipopolysaccharide-induced microtubule depolymerization via up-regulation of microtubule-associated protein 4 phosphorylation in human vascular endothelium.  |  Zhou, Z., et al. 2015. Surgery. 157: 590-8. PMID: 25633728
6. Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.  |  Li, L., et al. 2019. Burns Trauma. 7: 8. PMID: 30906793
7. Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics.  |  Ookata, K., et al. 1995. J Cell Biol. 128: 849-62. PMID: 7876309
8. Cellular microtubules heterogeneous in their content of microtubule-associated protein 4 (MAP4).  |  Chapin, SJ. and Bulinski, JC. 1994. Cell Motil Cytoskeleton. 27: 133-49. PMID: 7909279
9. A muscle-specific variant of microtubule-associated protein 4 (MAP4) is required in myogenesis.  |  Mangan, ME. and Olmsted, JB. 1996. Development. 122: 771-81. PMID: 8631255
10. Phosphorylation of microtubule-associated proteins MAP2 and MAP4 by the protein kinase p110mark. Phosphorylation sites and regulation of microtubule dynamics.  |  Illenberger, S., et al. 1996. J Biol Chem. 271: 10834-43. PMID: 8631898

The preferred MAP-4 antibody is MAP-4 Antibody (G-10): sc-390286.