Anticorps JAM-A (43) recommandé pour la détection de JAM-A d'origine human par WB et IP

Anticorps JAM-A (43): sc-135956

Anticorps JAM-A (43) est évalué 4.0 de 5 de 1.
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Fiches techniques

    • L'anticorps JAM-A 43 est un monoclonal IgG1 de souris fourni en 50 µg/0.5 ml
    • dirigé contre les acides aminés 126-237 de JAM-A d'origine human
    • recommandé pour la détection de JAM-A d'origine human par WB et IP
    • Voir JAM-A (J10.4): sc-53623 pour d'autres anticorps conjugués JAM-A, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for JAM-A Antibody (43). This work is in progress.
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I am using JAM-A (43): sc-135956 antibody for immunoprecipitation, how much antibody should I use?

Posée par: Dr Ninau Qelp
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 4 de 5 de par Robust signal in WB in human endothelial Robust signal in WB in human endothelial whole cell lysate. -SCBT QC
Date de publication: 2014-09-08
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