Anticorps insulin R beta (CT-3) recommandé pour la détection de insulin Rβ d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps insulin Rβ (CT-3): sc-57342

Anticorps insulin Rβ (CT-3) est évalué 4.3 de 5 de 18.
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Fiches techniques

    • Anti-L'anticorps insulin R beta CT-3 est un monoclonal IgG1 (chaîne légère kappa) L'Anticorps insulin Rβ fourni en 200 µg/ml
    • dirigé contre le C-terminus de insulin receptor de human
    • Anti-L'Anticorps insulin R beta (CT-3) est recommandé pour la détection de insulin Rβ d'origine mouse, rat et human par WB, IP, IF et IHC(P); non cross-reactif avec insulin-like growth factor (IGF)receptors
    • Anti-L'Anticorps insulin R beta (CT-3) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Voir m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nos recombinants vivement recommandés en alternative des réactifs IgG secondaires anti-souris conventionels.
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de insulin Rβ (CT-3): sc-57342.
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Is a blocking pepide available for the sc-57342 Insulin receptor antibody?

Posée par: PaulDA
Thank you for your question. There is no blocking peptide available for this specific antibody.
Répondue par: Technical Support
Date de publication: 2019-09-11

Can you provide information about the hybridoma cell line this antibody was produced from?

Posée par: KMitok
Thank you for your question. The specific clone name for this monoclonal antibody is CT-3. If you require additional information, please contact our Technical Service Department for assistance.
Répondue par: Technical Support
Date de publication: 2019-09-11

I have a clear band of approximately 45 KDa with this antibody. Do you know what it can be? Thank you so much!

Posée par: Jimena
Thank you for your question. We do not have any information regarding a 45 kDa sized band when using this antibody. Please contact our Technical Support Department for help with troubleshooting the antibody, or more detailed information about the expected molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Répondue par: Technical Support
Date de publication: 2018-11-22

In the data sheet , it was written the antigen is C-terminal , does the C-terminal region contain Tryosine domain?

Posée par: TANG QIAN
Thank you for your question. This antibody was made against a recombinant protein fragment including the C-terminal 100 amino acid residues of human insulin receptor (accession#P06213). There are tyrosine domains within the C-terminus but since the antibody has not been epitope mapped, I do not know if the exact epitope includes a Tyrosine. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Répondue par: Tech Service
Date de publication: 2017-09-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with insulin Rβ (CT-3): sc-57342 monoclonal antibody?

Posée par: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Répondue par: Technical Support 4
Date de publication: 2017-01-10
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Rated 4 de 5 de par Application of insulin Rβ Antibody for IHC and WB The insulin Rβ Antibody (CT-3) SC-57342 is suitable for intracellular localization studies of the insulin receptor in 3T3-L1 adipocytes at different stages of differentiation by immunofluorescence and confocal microscopy . The antibody can be also used for Western blot studies of protein extracts from 3T3-L1 adipocytes, including quantitative analyses.
Date de publication: 2018-09-13
Rated 5 de 5 de par Works great! Works at 1:50 dilution for immunofluorescence in hES cells
Date de publication: 2018-03-01
Rated 5 de 5 de par Works great for IHC in frozen mouse liver sections I used this at a 1:250 dilution on frozen mouse liver sections and it worked great! Did not work well in paraffin embedded sections.
Date de publication: 2018-01-03
Rated 4 de 5 de par nice band the antibody was tested in western blot on rat tissue samples and worked well
Date de publication: 2017-12-13
Rated 4 de 5 de par positive result in WB worked well for detection of insulin receptor from cell lysate
Date de publication: 2017-09-06
Rated 3 de 5 de par ISR b This antibody was used for Western blotting of MCF7 and T47D cell lysate。It did not work in T47D。It had some signal with a little background in MCF7.
Date de publication: 2017-06-15
Rated 4 de 5 de par relative high sensitivity show some cross reaction with IGF-1Rb, but generally ok to detect IRb
Date de publication: 2017-05-17
Rated 1 de 5 de par Confronto tra anticorpo poli e monoclonale A parità di proteine caricate (40ug) e condizioni di diluizione anticorpo (1:1000 in milk 2.5%) il nuovo anticorpo monoclonale (sc-57342) non funziona in modo soddisfacente rispetto al vecchio anticorpo policlonale (sc-711).
Date de publication: 2017-04-03
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