Anticorps Histone H1 (H-2) recommandé pour la détection de all histone H1 isoforms d'origine mouse, rat et human par WB, IP, IF et ELISA

Anticorps Histone H1 (H-2): sc-393358

Anticorps Histone H1 (H-2) est évalué 4.3 de 5 de 7.
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Fiches techniques

    • Anti-L'Anticorps Histone H1 (H-2) est un monoclonal de souris IgG1 λ L'Anticorps Histone H1, cité dans 19 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 22-47 près de N-terminus de Histone H1 d'origine human
    • Anti-L'Anticorps Histone H1 (H-2) est recommandé pour la détection de all histone H1 isoforms d'origine mouse, rat et human par WB, IP, IF et ELISA
    • Anti-L'Anticorps Histone H1 (H-2) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-393358 X, 200 µg/0.1 ml)
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Histone H1 (H-2): sc-393358.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Histone H1 Antibody (H-2) for WB applications. This reagent is now offered in a bundle with Histone H1 Antibody (H-2) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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When should the TransCruz reagent sc-393358 X be used instead of the standard form sc-393358?

Posée par: jenni
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Répondue par: Technical Support 7
Date de publication: 2017-01-13

What secondary is best to use with Histone H1 (H-2): sc-393358?

Posée par: jenni
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with Histone H1 (H-2):sc-393358, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Répondue par: Technical Support 7
Date de publication: 2017-01-13
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Rated 1 de 5 de par Not good for IF Weak signal for IF in hunam HT1080 cells at 1:100 dilution.
Date de publication: 2017-09-27
Rated 5 de 5 de par Good for WB Strong single band - worked well in nuclear fractionation WB.
Date de publication: 2017-09-13
Rated 5 de 5 de par Works great for Western Blot. Used at a concentration of 1:2000 for Western blot with mouse tissues. Worked very well, clean blot and a single band. I would recommend!
Date de publication: 2017-09-01
Rated 5 de 5 de par Superb !! Great product for western blot applications; strong signal
Date de publication: 2017-01-20
Rated 5 de 5 de par good in WB This Histone H1 antibody shows specific strong bands in WB.
Date de publication: 2017-01-12
Rated 4 de 5 de par Potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~32-33 kDa target protein in a HeLa/Jurkat cell lysate mix, but not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date de publication: 2016-11-10
Rated 5 de 5 de par Clean blot with single strong band at expected Clean blot with single strong band at expected size using A-431, LNCaP, HeLa and HL-60 whole cell lysates and Jurkat nuclear extract. -SCBT QC
Date de publication: 2014-07-05
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