GPRC5C Double Nickase Plasmid (h): sc-406870-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GPRC5C Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- GPRC5C Double Nickase Plasmid (h) and GPRC5C Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPRC5C. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GPRC5C Double Nickase Plasmid (h) | sc-406870-NIC | 20 µg | $410.00 | |||
GPRC5C Double Nickase Plasmid (h2) | sc-406870-NIC-2 | 20 µg | $410.00 |
GPRC5C (G protein-coupled receptor class C group 5 member C) is an orphan GPCR-like transmembrane protein implicated in regulating epithelial differentiation and context-dependent signal transduction. It is linked to processes downstream of receptor-mediated signaling, including modulation of MAPK and other pathways that influence proliferation, adhesion, and cellular stress responses. Expression of GPRC5C varies across tissue types and has been reported to change in multiple disease settings, supporting its use as a molecular handle to study altered signaling states. Experimental interrogation of GPRC5C helps clarify how GPCR-related networks shape cell identity and adaptive responses in human cellular models.
GPRC5C Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPRC5C locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPRC5C. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPRC5C function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPRC5C-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.