Anticorps GATA-1 (N6) recommandé pour la détection de GATA-1 d'origine mouse, rat et human par WB, IP, IF et IHC(P)

Anticorps GATA-1 (N6): sc-265

Anticorps GATA-1 (N6) est évalué 4.5 de 5 de 16.
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Fiches techniques

    • Anti-L'anticorps GATA-1 N6 est un monoclonal IgG2a L'Anticorps GATA-1, cité dans 293 publications, fourni en 200 µg/ml
    • dirigé contre la GATA-1 recombinante d'origine mouse
    • Anti-L'Anticorps GATA-1 (N6) est recommandé pour la détection de GATA-1 d'origine mouse, rat et human par WB, IP, IF et IHC(P)
    • Anti-L'Anticorps GATA-1 (N6) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-265 X, 200 µg/0.1 ml)
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de GATA-1 (N6): sc-265.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GATA-1 Antibody (N6). This work is in progress.
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Is sc-265 X also suitable for Western blotting?

Posée par: Kiki Chem
Thank you for your question. Yes, you can use sc-265 X for Western blotting. The only difference is that the "X" version of the antibody is 10x more concentrated in a buffer without gelatin (PBS + sodium azide only). Therefore, we recommend adjusting the recommended dilutions for Western blotting accordingly. For example, instead of using a 1:100 dilution, you could use a 1:1,000 dilution for Western blotting with the sc-265 X version of the antibody.
Répondue par: Technical Support
Date de publication: 2020-05-07

Does GATA-1 (N6) sc-265 antibody recognize the full and short isoform of GATA-1?

Posée par: Ricko
Thank you for your question. GATA-1 Antibody (N6) is a rat monoclonal IgG2a raised against recombinant GATA-1 of mouse origin. The exact epitope recognized by this monoclonal antibody has not been determined. We have not specifically tested this antibody for reactivity with different isoforms of GATA-1. We recommend you refer to some of the product citations using this antibody to see if anyone else has published this information previously.
Répondue par: Technical Support
Date de publication: 2019-12-03

Does GATA-1 (N6) sc-265 bind to N-terminal or C-terminal of GATA1? I want to detect N-terminal only. Thank you.

Posée par: Jesmine
Thank you for your question. This antibody, sc-265 GATA-1 (N6), maps to the N-terminus of GATA-1. If you have any further questions, please reach out to your local distributor. You can find their information here: https://www.scbt.com/scbt/resources/distributors
Répondue par: Tech Service
Date de publication: 2017-12-04

What is the recommended storage condition for GATA-1 (N6): sc-265?

Posée par: DefinitelyNotMatt
Thank you for your question. We recommend storing the antibody at 4° C.
Répondue par: Technical Support
Date de publication: 2017-03-27
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Rated 4 de 5 de par 免疫組織化学で良好な結果 免疫蛍光染色により精巣におけるセルトリ細胞のGATA-1を検出できた。 さらに、特異性も高く優れた抗体である。
Date de publication: 2021-01-29
Rated 4 de 5 de par 抗体小样很给力 买了一支常用抗体,赠送的小样,很慷慨,很好用。建议多搞这样的活动,有些分子不确定表达,就是试一把。这时候小样很重要。确定表达了,再买,减少浪费
Date de publication: 2020-06-28
Rated 5 de 5 de par Great Images! Used it in an EMSA setting to detect GATA-1 binding to DNA and got clear data. Totaly recommended!
Date de publication: 2019-10-05
Rated 5 de 5 de par IF很好用 我用在小鼠卵黄膜上血细胞的免疫荧光染色,1:200稀释很好用,背景很低,阳性信号强,是一个好抗体,推荐!
Date de publication: 2018-10-18
Rated 4 de 5 de par Works First purchased this antibody in 2012 and 3 years later, it stopped working despite different antigen retrieval methods. Many SC antibodies work well beyond their recommended use date, but this is not one of them. So re-ordered in 2018 and it works well. We used this antibody on PFA fixed, paraffin embedded mouse testis cut at 5um sections, for immuno-fluorescent staining. We used a pH6 antigen retrieval method in a PT-Link machine or pressure cooker. NOTE: The antibody does not work on bouin fixed tissues.
Date de publication: 2018-06-05
Rated 5 de 5 de par Good for WB, Co-IP, and ChIP This antibody is good for Western blot, co-IP and ChIP using mouse cells.
Date de publication: 2018-05-12
Rated 5 de 5 de par GATA-1 antibody I used the antibody for immunofluroscence staining and the antibody worked fine
Date de publication: 2017-09-22
Rated 5 de 5 de par Superb antibody Clear, specific western along with very strong ihc data
Date de publication: 2017-01-03
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