Anticorps GATA-1 (N1) recommandé pour la détection de GATA-1 d'origine mouse, rat et human par WB, IP et IF

Anticorps GATA-1 (N1): sc-266

Anticorps GATA-1 (N1) est évalué 5.0 de 5 de 10.
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Fiches techniques

    • L'anticorps GATA-1 N1 est un monoclonal IgG2a de rat, cité dans 65 publications, fourni en 200 µg/ml
    • raised against bacterially expressed murine GATA-1
    • Anti-L'Anticorps GATA-1 (N1) est recommandé pour la détection de GATA-1 d'origine mouse, rat et human par WB, IP et IF; inhibe la liaison de l'ADN dans les essais de retard sur gel
    • Anti-L'Anticorps GATA-1 (N1) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Réactif TransCruz pour une utilisation en Gel Supershift et ChIP (sc-266 X, 200 µg/0.1 ml)
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de GATA-1 (N1): sc-266.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for GATA-1 Antibody (N1). This work is in progress.
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Does GATA-1 (N1) sc-266 bind to N-terminal or C-terminal of GATA1? I want to detect C-terminal only. Thank you

Posée par: Double T
Thank you for your questin. GATA-1 Antibody (N1): sc-266 was raised against bacterially expressed murine GATA-1. No epitope mapping of this antibody has so far been performed, so we are not able to say whether it recognizes the N- or C-terminus or an internal region of the protein. Feel free to contact our Technical Service Department for more information on how to receive a free 10 µg sample of GATA-1 (N1): sc-266 if you would like to test the antibody.
Répondue par: Tech Support Europe
Date de publication: 2020-06-29

When should the TransCruz reagent sc-266 X be used instead of the standard form sc-266?

Posée par: TinTin
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Répondue par: Tech Service
Date de publication: 2017-01-31
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Rated 5 de 5 de par 免疫組織化学で良好な結果 免疫蛍光染色により精巣におけるセルトリ細胞のGATA-1を検出できた。 さらに、特異性も非常に高く優れた抗体である。
Date de publication: 2021-01-29
Rated 5 de 5 de par Nice band We used this antibody for a western blot and got a very clean band
Date de publication: 2016-12-23
Rated 5 de 5 de par This antibody worked well for IP This antibody worked well for IP, published with Cos, HPCs (human) and mouse lymphoma cells. -SCBT Publication Review
Date de publication: 2015-04-20
Rated 5 de 5 de par This GATA This GATA-1 antiobody (N1) was useful in immunohistochemistry using human hematopoietic cells. -SCBT Publication Review
Date de publication: 2015-04-16
Rated 5 de 5 de par Publishable data obtained using product sc Publishable data obtained using product sc-266 in immunohistochemistry on formalin fixed, paraffin embedded (FFPE) mouse gonads. -SCBT Publication Review
Date de publication: 2015-04-06
Rated 5 de 5 de par Works well in Western Blot using human hematopoietic, HPCs, erythroid, K-562, MEL, UT-7, Jurkat, HeLa, UT-7 5.3, U-937, HL-60 HEL and TF1 cell lines. -SCBT Publication Review
Date de publication: 2015-04-03
Rated 5 de 5 de par Human MEL and umbelical vein endothelial Human MEL and umbelical vein endothelial cells were used in ChIP application successfully and results published. -SCBT Publication Review
Date de publication: 2015-02-20
Rated 5 de 5 de par Antibody was used successfully in EMSA using Antibody was used successfully in EMSA using mouse C19 MEL and human Jurkat, HeLa, K-562, ovarian and breast cells. -SCBT Publication Review
Date de publication: 2015-01-25
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