Anticorps Cytokeratin 19 (A-3) recommandé pour la détection de Cytokeratin 19 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Cytokeratin 19 (A-3): sc-376126

Anticorps Cytokeratin 19 (A-3) est évalué 4.2 de 5 de 14.
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Fiches techniques

    • Anti-L'anticorps Cytokeratin 19 A-3 est un monoclonal IgG3 κ L'Anticorps Cytokeratin 19, cité dans 27 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 7-29 au niveau N-terminus de Cytokeratin 19 d'origine human
    • recommandé pour la détection de Cytokeratin 19 d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA; également réactif avec d'autres espèces, dont equine
    • disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; à la phycoérythrine, à la fluorescéine, à l'Alexa Fluor® 488 ou à l'Alexa Fluor® 647 pour IF, IHC(P) et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Cytokeratin 19 (A-3): sc-376126.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for Cytokeratin 19 Antibody (A-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with Cytokeratin 19 Antibody (A-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Me gustaría saber si este anticuerpo funciona para IHC-P en perro (mucosa oral)

Posée par: Sobolaira
Cytokeratin 19 Anticuerpo (A-3): sc-376126 esta recomendado para detectar Cytokeratin 19 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; también reactivo con otras especies, incluyendo equine. Lo no hemos probado en perro. Thank you for your question. Cytokeratin 19 Antibody (A-3): sc-376126 is only recommended for detection of Cytokeratin 19 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. It has not been tested on canine tissues or cells.
Répondue par: Tech Support Europe
Date de publication: 2021-01-25

How can Cytokeratin 19 Antibody (A-3): sc-376126 be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Posée par: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody is currently available conjugated to phycoerythrin (sc-376126 PE), fluorescein (sc-376126 FITC), Alexa Fluor® 488 (sc-376126 AF488) or Alexa Fluor® 647 (sc-376126 AF647)
Répondue par: Technical Support 14
Date de publication: 2017-01-09
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Rated 1 de 5 de par Did not work in rat tongue Keratin19 labels taste bud in rat tongue but this one is staining only few cell in the taste bud with a high background.
Date de publication: 2018-01-19
Rated 4 de 5 de par work fine in IHC,but unknown in IF The antibody work in IF and IHC at 1:100 for mouse primary liver cancer cells, hepa1-6 cells and 3T3 cells, but the FITC is negative through stained.Maybe these cells essentially can't express the protein or other reasons. After we searched the certain cell lines of full CK19 expression,namely hepaG2, BUT we are sorry to be note that the sample is over. (The photo of IHC work fine, but we didn't upload resulted by the store location of photos.) 3T3 cells for negative group.
Date de publication: 2017-10-24
Rated 5 de 5 de par Very good for FFPE This antibody was used on formalin-fixed paraffin-embedded human carcinomas at a dilution of 1:30 for 15 minutes. Staining was very good.
Date de publication: 2017-08-10
Rated 1 de 5 de par Doesn't work for immunofluorescence on mouse cells Used on mouse ovarian epithelial cells at 1/100 overnight at 4C then 1/500 goat α-mouse secondary for 1H at room temp. Low signal to noise ratio.
Date de publication: 2017-03-24
Rated 4 de 5 de par Cytokeratin 19 We used Cytokeratin 19 as a marker of HepG2 cells with IF and got nice stainning
Date de publication: 2017-02-16
Rated 5 de 5 de par Outstanding for multiple applications !! Great antibody for WB, IHC and IF studies; much better than polyclonal alternatives
Date de publication: 2017-01-11
Rated 5 de 5 de par Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, equine, and human samples.
Date de publication: 2016-12-29
Rated 4 de 5 de par Good product. I recieved it 1 mouth ago and used it for IF,1:100,not bad.
Date de publication: 2016-12-22
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