Anticorps C/EBP beta (47A1) recommandé pour la détection de C/EBP β d'origine mouse, rat et human par WB et IP

Anticorps C/EBP β (47A1): sc-56637

Anticorps C/EBP β (47A1) est évalué 4.3 de 5 de 3.
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Fiches techniques

    • L'anticorps C/EBP beta 47A1 est un monoclonal IgG1 de souris, cité dans 10 publications, fourni en 50 µg/0.5 ml
    • dirigé contre les acides aminés 1-271 de C/EBP β d'origine human
    • recommandé pour la détection de C/EBP β d'origine mouse, rat et human par WB et IP
    • Voir C/EBP β (H-7): sc-7962 pour d'autres anticorps conjugués C/EBP β, dont AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 et 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for C/EBP β Antibody (47A1). This work is in progress.
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I'm trying to find an antibody that detects both the Lap and Lap* isoforms, but NOT Lip. Can you tell me all the isoforms detected by C/EBP β Antibody (47A1)? Can you recomend an IF/ICC antibody that only detects the Lap/Lap* isoforms? Thanks

Posée par: Glory
Thank you for your question. Please contact your local distributor or technical service for further assistance.
Répondue par: Tech Service 1
Date de publication: 2021-01-22

I am using C/EBP β (47A1): sc-56637 antibody for immunoprecipitation, how much antibody should I use?

Posée par: Trav11
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Répondue par: Technical Support
Date de publication: 2017-03-24
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Rated 4 de 5 de par Worked well for WB with Jurkat whole cell lysates Worked well for WB with Jurkat whole cell lysates. -SCBT QC
Date de publication: 2015-04-28
Rated 4 de 5 de par Great WB data in HeLa and Hep G2 nuclear extracts Great WB data in HeLa and Hep G2 nuclear extracts. -SCBT QC
Date de publication: 2013-06-21
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