Anticorps Annexin VI (E-5) recommandé pour la détection de Annexin VI d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA

Anticorps Annexin VI (E-5): sc-271859

Anticorps Annexin VI (E-5) est évalué 4.9 de 5 de 8.
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Fiches techniques

    • Anti-L'anticorps Annexin VI E-5 est un monoclonal IgG1 κ L'Anticorps Annexin VI, cité dans 2 publications, fourni en 200 µg/ml
    • spécifique d'un épitope situé entre les acides aminés 5-27 au niveau N-terminus de Annexin VI d'origine human
    • Anti-L'Anticorps Annexin VI (E-5) est recommandé pour la détection de Annexin VI d'origine mouse, rat et human par WB, IP, IF, IHC(P) et ELISA
    • Anti-L'Anticorps Annexin VI (E-5) est disponible conjugué à l'agarose pour IP; à l'HRP pour WB, IHC(P) et ELISA; et soit à la phycoerythrin ou FITC pour IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 ou Alexa Fluor® 647 pour WB (RGB), IF, IHC(P) et FCM
    • aussi disponible conjugué à l'Alexa Fluor® 680 ou Alexa Fluor® 790 pour WB (NIR), IF et FCM
    • Contactez notre Service Technique (ou votre Distributeur local) pour plus d'informations pour recevoir un échantillon GRATUIT de 10 µg de Annexin VI (E-5): sc-271859.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for Annexin VI Antibody (E-5) for WB and IHC(P) applications. This reagent is now offered in a bundle with Annexin VI Antibody (E-5) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What application is the blocking peptide sc-271859 P appropriate for?

Posée par: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Répondue par: Technical Support
Date de publication: 2017-02-27
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Rated 5 de 5 de par Ottimo funzionamento!!! L’anticorpo anti-Annexin VI (sc-271859), richiesto con l’Ordine n° 535 dell’11/11/2021 e arrivato il giorno 23/11/2021, è stato testato su un lisato di cellule HeLa, ottenuto tramite omogenizzazione manuale in una soluzione di PBS-0.1% Igepal contenente un cocktail di inibitori di proteasi. Aliquote di lisato da 2 μg, 5 μg, 10 μg e 20 μg di proteine sono state caricate su un gel di poliacrilammide al 10% e sottoposte a Western Blotting. La membrana così ottenuta è stata sottoposta a blocking (in una soluzione al 5% di non-fat dried milk in TBS-0.05% TWEEN 20) e, successivamente, incubata con l’anticorpo primario sopra riportato (1:1000 vol/vol). La membrana è poi stata incubata con un anticorpo secondario anti-mouse (1:1500 vol/vol) coniugato con perossidasi di rafano. I segnali di chemiluminescenza sono stati rivelati tramite il sistema di imaging digitale Amersham Imager 680 (GE Healthcare Life Sciences). Come mostrato nell’immagine di seguito riportata, l’anticorpo produce segnali in corrispondenza del peso molecolare dell’Annessina VI in maniera concentrazione dipendente.
Date de publication: 2021-11-25
Rated 5 de 5 de par This antibody is good for WB and IP I got this antibody few month before, it is good for both WB and IP
Date de publication: 2017-11-20
Rated 5 de 5 de par works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date de publication: 2017-09-20
Rated 4 de 5 de par WORKED WELL I received this antibody as a free trial sample and used for WB and Immunoprecipitation. It worked well.
Date de publication: 2017-09-11
Rated 5 de 5 de par Distinct band observed via WB using product sc Distinct band observed via WB using product sc-271859 using HeLa whole cell lysate. -SCBT QC
Date de publication: 2015-04-12
Rated 5 de 5 de par Good specificity shown by using transfected Good specificity shown by using transfected and non-transfected 293T cells for over-expression of the human Annexin VIII protein via Western Blot analysis. -SCBT QC
Date de publication: 2015-03-29
Rated 5 de 5 de par Positive detection of endogenous Annexin Positive detection of endogenous Annexin VIII in JAR cells by WB analysis. -SCBT QC
Date de publication: 2015-03-21
Rated 5 de 5 de par Produced excellent immunofluorescence membrane Produced excellent immunofluorescence membrane and cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date de publication: 2015-02-26
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