VEGF-C Anticuerpo (E-6) recomendado para detectarprecursor and mature VEGF-C de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

VEGF-C Anticuerpo (E-6): sc-374628

VEGF-C Anticuerpo (E-6) clasificado 4.0 de 5 por 12.
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Ficha técnica

    • Anti-VEGF-C Anticuerpo E-6 es un monoclonal de ratón IgG2a κ VEGF-C Anticuerpo, ver las 18 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 103-137 cerca del N-terminus de VEGF-C oe orgiendf humanorigin
    • VEGF-C Anticuerpo (E-6) es recomendado para detectar precursor and mature VEGF-C de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • VEGF-C Anticuerpo (E-6) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de VEGF-C (E-6): sc-374628.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for VEGF-C Antibody (E-6) for WB and IHC(P) applications. These reagents are now offered in bundles with VEGF-C Antibody (E-6) (see ordering information below).
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Does this antibody have any neutralisation or inhibitory potential in any cell-based assays?

Preguntado por: Steven91
Thank you for your question. No, this antibody has not been tested for any neutralization or inhibitory use, so we do not have that information. It is recommended for detection of VEGF-C via WB, IP, PAR and ELISA.
Respondida por: Tech Service
Fecha de publicación: 2019-01-14

PMID: # 25796380, This Manuscript has not shown VEGFC expression by western blot. Please correct me if I am wrong.

Preguntado por: sdas128
Thank you for your question. This journal article is listed as a product citation for our mouse monoclonal antibody, VEGF-C (E-6): sc-374628, because this specific product catalog number and clone name were referenced in the materials and methods of the article. "Immunodetection of VEGF...was done using mouse monoclonal anti-VEGF antibody (1:1000, E-6, sc-374628; Santa Cruz Biotech, Santa Cruz...".
Respondida por: Technical Support
Fecha de publicación: 2017-05-15

What is the recommended secondary reagent for VEGF-C Antibody (E-6): sc-374628 in Western blot when working with human samples?

Preguntado por: Trav11
Thank you for your question. We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-8301 option 2, emailing [email protected], or using the Live Chat function on our website.
Respondida por: Technical Support 7
Fecha de publicación: 2017-02-03
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Rated 1 de 5 por de Not as good as expected No signal on WB at all at a dilution of 1 :250 on mouse lung lysates; incubated O/N at 4oC
Fecha de publicación: 2021-02-18
Rated 4 de 5 por de works well for WB and IF Shows a specific signal in WB (dilution 1:200 in BSA/TBS-T) at 40kDa for HEp-2, RKO and LoVo whole cell Lysates. When used in IF, the cells (HEp-2) were fixed in 2% formaldehyde and the dilution was adjusted to 1:50 - IF signal was cytoplasmic but still slightly diffuse
Fecha de publicación: 2020-01-09
Rated 4 de 5 por de Works well on lymphatic staining This antibody works well on lymphatic staining. Beautiful macrophages staining.
Fecha de publicación: 2019-10-09
Rated 5 de 5 por de Excelent antibody for western blotting I obtained really strong signal by western blotting, using a 1:500 dilution of this antibody in glioblastoma cell lines
Fecha de publicación: 2019-06-27
Rated 5 de 5 por de great features Strip is very noticeable, quality is very good,thank you very much
Fecha de publicación: 2017-09-05
Rated 1 de 5 por de unexpected protein size I tested sample with the lysates from mouse thymic epithelial cell lines and got non-specific band around 20kda. Conditions of WB ; 4-20% SDS-PAGE, Wet transfer (400mA/2hr), 1:200 primary antibody in 5% TBST.
Fecha de publicación: 2017-08-31
Rated 5 de 5 por de Great IF image We used this antibody for IF staining and got a very clean image showing great HeLa cells
Fecha de publicación: 2017-01-09
Rated 5 de 5 por de Highly potent and very specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~40/80 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three other very strongly cross-reactive proteins.
Fecha de publicación: 2016-11-10
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