TSSK 1 CRISPR/Cas9 KO Plasmid (m): sc-423531
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TSSK 1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TSSK 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TSSK 1 CRISPR/Cas9 KO Plasmid (m) | sc-423531 | 20 µg | $397.00 |
Overview
Tssk1 encodes testis-specific serine/threonine kinase 1 (TSSK1), a germ cell–restricted kinase that functions in post-meiotic spermatogenesis. TSSK1 contributes to sperm differentiation processes such as chromatin remodeling, flagellar assembly, and maturation of spermatids through phosphorylation-dependent signaling in the testis. Disruption of TSSK family kinase activity has been linked to impaired sperm development and male subfertility/infertility phenotypes in model systems. As a mouse gene with highly enriched testicular expression, Tssk1 is commonly studied to define kinase-regulated pathways controlling germ cell development and reproductive fitness.
TSSK 1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tssk1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tssk1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tssk1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TSSK 1 protein expression.
This CRISPR knockout system enables efficient generation of Tssk1-deficient cell models for investigation of TSSK 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tssk1 exon(s) critical for TSSK 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tssk1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TSSK 1 CRISPR/Cas9 KO Plasmid (m) and TSSK 1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tssk1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TSSK 1 HDR Plasmid (m) and TSSK 1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tssk1 homology arms to support homology-directed repair at defined Tssk1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.