TCR delta Anticuerpo (H-41) recomendado para detectar TCR δ de human origen, mediante WB, IP y ELISA

TCR δ Anticuerpo (H-41): sc-100289

TCR δ Anticuerpo (H-41) clasificado 4.7 de 5 por 6.
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Ficha técnica

    • Anti-TCR delta Anticuerpo H-41 es un monoclonal de ratón IgG1 κ TCR δ Anticuerpo, ver las 23 publicaciones, proporcionado como 100 µg/ml
    • producido contra una proteina recombinante TCR δ de origen human
    • recomendado para detectar TCR δ de human origen, mediante WB, IP y ELISA
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de TCR δ (H-41): sc-100289.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for TCR δ Antibody (H-41) for WB applications. This reagent is now offered in a bundle with TCR δ Antibody (H-41) (see ordering information below).
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As per your data, this clone detects TCR delta chain protein in HeLa and K562 cells which are not gamma-delta T cells. Are these cell lines transfected with delta chain expressing constructs ?

Preguntado por: rnahar
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801. ext.1, allowing for a more interactive discussion of this and other related questions.
Respondida por: Blake J
Fecha de publicación: 2021-10-18

Does the antibody bind to constant region of delta chain? what's the epitope ?

Preguntado por: rnahar
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801. ext.1, allowing for a more interactive discussion of this and other related questions.
Respondida por: Blake J
Fecha de publicación: 2021-10-18

Does this antibody work in mouse tissues?

Preguntado por: Tero
Thank you for your question. Anti-TCR δ Antibody (H-41): sc-100289 is only recommended for detection of TCR δ of human origin by WB, IP and ELISA.
Respondida por: Tech Support Europe
Fecha de publicación: 2021-09-13

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Preguntado por: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Service 11
Fecha de publicación: 2017-02-03
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Rated 5 de 5 por de パラフィン切片の免疫染色で綺麗に染まります 通常のパラフィン切片で、EDTAph8.0で圧力鍋を使用し、陽性コントロールがとてもよく染まりました。
Fecha de publicación: 2021-04-08
Rated 5 de 5 por de Very good TCRgd+ cell staining on FFPE After TCR gamma.20 (from Thermo Scientific) being discontinued, finally I've found in Santa Cruz Biotechnology TCR delta a solid antibody useful to recognize TCRgd cells in formalin-fixed paraffin embedded sample using immunohistochemistry. I've stained sections from tonsil and small intestine with a range of dilution from 1:25 to 1:250 and I've achieved strong and tidy stainings in all dilutions tested. Also antigen unmasking is very tolerant. It works using EDTA pH8.0 or Citrate pH 6.0 in microwave or water bath. I'm very satisfied from the results
Fecha de publicación: 2020-03-12
Rated 5 de 5 por de Excellent staining for IHC on FFPE tissues I successfully used this product for immunohistochemistry on 4 micron sections of FFPE tonsil tissues and FFPE cell block of a bone marrow blood clot. I got excellent results using 1:50-1:100 dilution for 1 hour incubation at room temperature manually or using the Ventena Benchmark Ultra autostainer. The best results were obtained using EDTA pH9 in a steamer then placed on the autostainer, or alternately using CC1 at 99 degrees C for 36 minutes. Ventena Ultraview/DAB was used for the detection system.
Fecha de publicación: 2018-06-19
Rated 5 de 5 por de Clean and Great expression of TCR Delta. I struggled for over a year to find a replacement for TCR Gamma, which was discontinued by Thermo last year. This is a great replacement for it. I can't upload a TIFF image, but the examples that I wanted to upload were from a Spleen and Colon. The retrieval for Leica Bond III was Hier2 for 40 minutes with 15 minutes of antibody incubation (modified F Protocol). Dilution that worked best was 1:200 (using Leica's diluent).
Fecha de publicación: 2017-12-20
Rated 5 de 5 por de Good for human detection Positive signal obtained at predicted molecular weight in WB for TCR delta.
Fecha de publicación: 2017-02-07
Rated 3 de 5 por de Faint band at correct MW in HeLa whole cell lysate Faint band at correct MW in HeLa whole cell lysate. -SCBT QC
Fecha de publicación: 2013-12-12
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