TCR beta Anticuerpo (G-11) recomendado para detectar TCR β de human origen, mediante WB, IP, IF, IHC(P) y ELISA

TCR β Anticuerpo (G-11): sc-5277

TCR β Anticuerpo (G-11) clasificado 4.9 de 5 por 10.
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Ficha técnica

    • TCR β Anticuerpo (G-11) es un monoclonal de ratón IgG1 κ, ver las 10 publicaciones, proporcionado como 200 µg/ml
    • raised against the constant region of TCR β of human origin
    • TCR beta Anticuerpo (G-11) es recomendado para detectar TCR β de human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • TCR beta Anticuerpo (G-11) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de TCR β (G-11): sc-5277.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for TCR β Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with TCR β Antibody (G-11) (see ordering information below).
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Does it recognize mouse TCR beta or only human

Preguntado por: Maria Teresa
Thank you for your question. This antibody has not been tested for mouse and rat use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Respondida por: Tech Service
Fecha de publicación: 2019-03-01

I tried this Ab for IF with dilution 1:50 on 4% PFA fixed Jurkat cells but I didn't see the specific staining. How should I troubleshoot it?

Preguntado por: KanyaratP
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Respondida por: Technical Support
Fecha de publicación: 2019-01-14

Any idea how much to use TCR β Antibody (G-11) for Flow Cytometry? I tested at final staining concentration 5ug/ml and failed to see any positive stain in purified human T-cells.

Preguntado por: JonathanH
Thank you for your question. Below is a link to our recommended protocol for flow cytometry. Please contact our Technical Service Department if you would like help troubleshooting the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Respondida por: Technical Support
Fecha de publicación: 2017-10-12

Does TCR β (G-11): sc-5277 react with canine samples?

Preguntado por: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Respondida por: Technical Support 15
Fecha de publicación: 2017-01-16

Why is TCR β (G-11): sc-5277 the recommended alternative to the now discontinued TCR β (H-197): sc-9101?

Preguntado por: Trav11
Thank you for your question. The monoclonal primary antibody TCR β (G-11): sc-5277 offers a higher degree of specificity and essentially eliminates all lot-to-lot variation. It is also warrantied for all of the same applications as the discontinued polyclonal antibody: WB, IP, IF, and IHC(P). If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Respondida por: Technical Service 11
Fecha de publicación: 2017-01-16
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Rated 5 de 5 por de 还不错 虽然订购过程很郁闷,但是真正发货还是很快到货的。使用的时候基本上按照说明书的稀释比使用,基本能满足要求
Fecha de publicación: 2021-04-06
Rated 5 de 5 por de IHC I do IHC with this antibody on human brain sections. I got excellent specific staining of interneurons without any background. The antibody dilution: 1:200.
Fecha de publicación: 2017-08-19
Rated 5 de 5 por de The sc-5277 TCR beta antibody has good specificity, it detect this sample from dilution 1:500, clean background.
Fecha de publicación: 2017-05-07
Rated 5 de 5 por de IHC-P This antibody could be used to do IHC-P in human samples.
Fecha de publicación: 2017-02-10
Rated 5 de 5 por de Outstanding product !! Better than polyclonal alternative for WB, IF and IHC applications
Fecha de publicación: 2017-01-20
Rated 5 de 5 por de Good for IHC and IF This TCR beta antibody shows good staining for IF and IHC in human samples.
Fecha de publicación: 2017-01-12
Rated 5 de 5 por de Robust bands in western blot analysis of Robust bands in western blot analysis of TCR expression in MOLT-4 and CCRF-CEM whole cell lysates. -SCBT QC
Fecha de publicación: 2015-06-30
Rated 5 de 5 por de Great IF images using this antibody with methanol Great IF images using this antibody with methanol-fixed CCRF-CEM cells -SCBT QC
Fecha de publicación: 2015-06-16
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