Skeletal Muscle Myosin Anticuerpo (S58) recomendado para detectarslow fiber–specific Skeletal Muscle Myosin heavy chain de mouse, rat, human y avian origen, mediante WB, IP y IF

Skeletal Muscle Myosin Anticuerpo (S58): sc-32733

Skeletal Muscle Myosin Anticuerpo (S58) clasificado 4.2 de 5 por 5.
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Ficha técnica

    • Skeletal Muscle Myosin Anticuerpo (S58) es un monoclonal de ratón IgA κ, ver las 6 publicaciones, proporcionado como 200 µg/ml
    • raised against Myosin purified from upper leg muscle of embryonic day 19 white Leghorn chicken origin
    • recomendado para detectarslow fiber–specific Skeletal Muscle Myosin heavy chain de mouse, rat, human y avian origen, mediante WB, IP y IF
    • Ver Skeletal Muscle Myosin (F59): sc-32732 para Skeletal Muscle Myosin anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Skeletal Muscle Myosin (S58): sc-32733.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for Skeletal Muscle Myosin Antibody (S58) for WB applications. This reagent is now offered in a bundle with Skeletal Muscle Myosin Antibody (S58) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using Skeletal Muscle Myosin (S58): sc-32733?

Preguntado por: jerojero
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Excellent! This antibody works great in western blot analysis of Skeletal Muscle Myosin expression in rat skeletal muscle tissue extract.
Fecha de publicación: 2017-01-19
Rated 5 de 5 por de Good antibody for WB The Skeletal Muscle Myosin Antibody (S58): sc-32732 provides excellent Western blot data showing clear band with minimal background.
Fecha de publicación: 2017-01-13
Rated 3 de 5 por de used in western blotting-satisfactory! We got this antibody on trial basis from our sales rep Korey Griffin to use as a marker for differentiated L6 cell lysates. No bands were seen in differentiated L6 cells but human skeletal muscle tissue lysates showed decent bands at the expected molecular weight.
Fecha de publicación: 2016-11-04
Rated 4 de 5 por de Western blot publication with mouse C2C12 Western blot publication with mouse C2C12. -SCBT Publication Review
Fecha de publicación: 2015-01-22
Rated 4 de 5 por de Worked well for Western Worked well for Western, single clean band in rat skeletal muscle tissue extract. -SCBT QC
Fecha de publicación: 2014-12-10
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