Skeletal Muscle Myosin Anticuerpo (F59) recomendado para detectarfast fiber–specific skeletal muscle myosin heavy chain de mouse, rat, human y avian origen, mediante WB, IP, IF y IHC(P)

Skeletal Muscle Myosin Anticuerpo (F59): sc-32732

Skeletal Muscle Myosin Anticuerpo (F59) clasificado 4.8 de 5 por 10.
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Ficha técnica

    • Anti-Skeletal Muscle Myosin Anticuerpo F59 es un monoclonal de ratón IgG1 κ Skeletal Muscle Myosin Anticuerpo, ver las 41 publicaciones, proporcionado como 200 µg/ml
    • raised against Myosin purified from pectoralis muscle of adult white Leghorn chicken origin
    • Skeletal Muscle Myosin Anticuerpo (F59) es recomendado para detectar fast fiber–specific skeletal muscle myosin heavy chain de mouse, rat, human y avian origen, mediante WB, IP, IF y IHC(P)
    • Skeletal Muscle Myosin Anticuerpo (F59) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Skeletal Muscle Myosin (F59): sc-32732.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for Skeletal Muscle Myosin Antibody (F59) for WB and IHC(P) applications. These reagents are now offered in bundles with Skeletal Muscle Myosin Antibody (F59) (see ordering information below).

Does this antibody detect any specific myosin heavy chain? Such as MYH1, MYH6, MYH7, MYH15, MYH2, MYH3, MYH4 etc?

Preguntado por: Eric Spinetti
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. This clone is recommended for the detection of fast fiber–specific skeletal muscle myosin heavy chain. The exact myosin heavy chain genes/proteins that are targeted are not known.
Respondida por: Technical Support
Fecha de publicación: 2019-09-20

I am interested in performing a immunoprecipitation of MYH6 using this antibody. However, I don't see any validation of this technique in your website or in any cited papers. Do you have any data demonstrating the efficiency of F59 (sc-32732) for IP?

Preguntado por: Anónimo
This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Respondida por: Tech Service
Fecha de publicación: 2019-09-20

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Preguntado por: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Respondida por: Technical Support 15
Fecha de publicación: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Preguntado por: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Support 15
Fecha de publicación: 2017-01-11
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Rated 4 de 5 por de Good signal This antibody works well on human and mouse samples in WB
Fecha de publicación: 2018-08-01
Rated 4 de 5 por de Great features! The antibody is work well in WB at 1:100 of C2C12 cells.
Fecha de publicación: 2017-08-31
Rated 5 de 5 por de good WB dilution 1/1000, mouse heart tissue detected clean band
Fecha de publicación: 2017-05-09
Rated 5 de 5 por de Sehr gut Application in mouse cells, very clear immunolabelling.
Fecha de publicación: 2017-04-05
Rated 5 de 5 por de Outstanding Strong western blot data, low background; nice IHC staining
Fecha de publicación: 2017-01-29
Rated 5 de 5 por de Positive result in WB I bought it for years,tested it in WB with pig dorsal muscles,it is very wonderful
Fecha de publicación: 2016-12-13
Rated 5 de 5 por de Western blot and IF publications using mouse Western blot and IF publications using mouse C2C12 myoblasts. -SCBT Publication Review
Fecha de publicación: 2015-03-12
Rated 5 de 5 por de Publishable IHC data in mouse cardiomyocytes Publishable IHC data in mouse cardiomyocytes. -SCBT Publication Review
Fecha de publicación: 2015-02-08
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