SGK3 Anticuerpo (C-6) recomendado para detectarSGK3 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

SGK3 Anticuerpo (C-6): sc-166847

SGK3 Anticuerpo (C-6) clasificado 4.6 de 5 por 5.
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Ficha técnica

    • Anti-SGK3 Anticuerpo C-6 es un monoclonal de ratón IgG2b κ SGK3 Anticuerpo, ver las 5 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 67-145 localizados cerca del N-terminus de SGK3 de origen human
    • SGK3 Anticuerpo (C-6) es recomendado para detectar SGK3 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • SGK3 Anticuerpo (C-6) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de SGK3 (C-6): sc-166847.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for SGK3 Antibody (C-6) for WB and IHC(P) applications. These reagents are now offered in bundles with SGK3 Antibody (C-6) (see ordering information below).

I am using SGK3 (C-6): sc-166847 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-25
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Rated 4 de 5 por de Good antibody The antibody detects a very strong band near 50-kDa and another strong band near 60-kDa which is decreased by SGK3 siRNA.
Fecha de publicación: 2017-09-11
Rated 4 de 5 por de Potent and quite specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~65+53 kDa target proteins in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, but slightly higher backgrounds.
Fecha de publicación: 2016-11-10
Rated 5 de 5 por de Excellent immunoperoxidase cytoplasmic and Excellent immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Fecha de publicación: 2015-03-30
Rated 5 de 5 por de Positive western blot detection of SGK3 expression Positive western blot detection of SGK3 expression in A-375 whole cell lysate. -SCBT QC
Fecha de publicación: 2015-02-02
Rated 5 de 5 por de Strong signal in immunofluorescence staining Strong signal in immunofluorescence staining of methanol-fixed HeLa cells showed cytoplasmic localization. -SCBT QC
Fecha de publicación: 2015-01-17
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