SGK Anticuerpo (H-4) recomendado para detectarSGK de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

SGK Anticuerpo (H-4): sc-28338

SGK Anticuerpo (H-4) clasificado 5.0 de 5 por 9.
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Ficha técnica

    • SGK Anticuerpo (H-4) es un monoclonal de ratón IgG2b κ, ver las 12 publicaciones, proporcionado como 200 µg/ml
    • specific for an epitope mapping between 400-425 containing Ser 422 of SGK of human origin
    • SGK Anticuerpo (H-4) es recomendado para detectar SGK de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • SGK Anticuerpo (H-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de SGK (H-4): sc-28338.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) is the preferred secondary detection reagent for SGK Antibody (H-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with SGK Antibody (H-4) (see ordering information below).

Does this antibody bind to Ser422-phosphorylated as well as unphosphorylated SGK1?

Preguntado por: asdf123
Thank you for your question. SGK Antibody (H-4): sc-28338 recognizes both phosphorylated and non-phosphorylated SGK protein and does not distinguish between different modifications.
Respondida por: Technical Support Europe
Fecha de publicación: 2020-10-08

What is the AC conjugate used for?

Preguntado por: ecelbarc
Thank you for your question. The agarose conjugated form of the antibody is usually used for immunoprecipitation (IP) applications.
Respondida por: Tech Service
Fecha de publicación: 2019-01-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Preguntado por: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Service 11
Fecha de publicación: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with SGK (H-4): sc-28338?

Preguntado por: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Service 11
Fecha de publicación: 2017-01-13
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Rated 5 de 5 por de Specific and Sensitive We used it at 1:300, strip clear, strong reaction.i`s nice
Fecha de publicación: 2017-05-12
Rated 5 de 5 por de Good operation Western blot analysis of human recombinant SGK, obtained strong bands
Fecha de publicación: 2017-04-19
Rated 5 de 5 por de Superb!! Outstanding product for IHC studies; also great WB !!
Fecha de publicación: 2017-01-18
Rated 5 de 5 por de Works Great This is a great antibody recommended for detection of SGK of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Fecha de publicación: 2016-12-23
Rated 5 de 5 por de Produced great immunoperoxidase cytoplasmic Produced great immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human gall bladder tissue. -SCBT QC
Fecha de publicación: 2015-04-14
Rated 5 de 5 por de Clean WB blot with single strong band in Clean WB blot with single strong band in human recombinant SGK. -SCBT QC
Fecha de publicación: 2015-02-22
Rated 5 de 5 por de Publishable immunostaining in paraffin embedded Publishable immunostaining in paraffin embedded small cell lung cancer. -SCBT Publication Review
Fecha de publicación: 2015-02-04
Rated 5 de 5 por de Positive detection of endogenously expressed Positive detection of endogenously expressed SGK protein in PC-12 whole cell lysate through analysis by Western Blot. -SCBT QC
Fecha de publicación: 2015-01-22
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