SAP 155 Anticuerpo (G-9) recomendado para detectarSAP 155 de mouse, rat y human origen, mediante WB, IP, IF y ELISA

SAP 155 Anticuerpo (G-9): sc-514494

SAP 155 Anticuerpo (G-9) clasificado 1.0 de 5 por 1.
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Ficha técnica

    • SAP 155 Anticuerpo G-9 es un monoclonal de ratón mouse IgG1 proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 7-26 en el N-terminus de SAP 155 de origen human
    • recomendado para detectarSAP 155 de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for SAP 155 Antibody (G-9) for WB applications. These reagents are now offered in bundles with SAP 155 Antibody (G-9) (see ordering information below).
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What application is the blocking peptide sc-514494 P appropriate for?

Preguntado por: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-27
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Rated 1 de 5 por de Don't waste your time I used this in an IP experiment against nuclear extract made with HeLa cells. The membrane for the IP experiment is immunoblot against the same antibody. The resultant blot is of very poor quality. No bands in any of the lanes (input, elution, etc) corresponding to 155kDa are visible. Only a band around 50kDa is visible across all lanes.
Fecha de publicación: 2016-11-30
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