Ribosomal Protein L23a Anticuerpo (3E11) recomendado para detectarRibosomal Protein L23a de mouse, rat y human origen, mediante WB, IP, IF y ELISA

Ribosomal Protein L23a Anticuerpo (3E11): sc-517097

Ribosomal Protein L23a Anticuerpo (3E11) clasificado 4.0 de 5 por 1.
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5
0
4
1
3
0
2
0
1
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Ficha técnica

    • Anti-Ribosomal Protein L23a Anticuerpo 3E11 es un monoclonal de ratón IgG2a (kappa light chain) Ribosomal Protein L23a Anticuerpo proporcionado como 100 µg/ml
    • raised against amino acids 59-156 representing partial length Ribosomal Protein L23a of human origin
    • recomendado para detectarRibosomal Protein L23a de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • Ver m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), nuestras altamente recomendadas alternativa a los anticuerpos secundarios convencionales, anti-IgG de ratón.
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Ribosomal Protein L23a (3E11): sc-517097.

I am using Ribosomal Protein L23a (3E11): sc-517097 for immunofluorescence, what dilution of this antibody should I use?

Preguntado por: TinTin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Technical Support
Fecha de publicación: 2017-03-24
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Rated 4 de 5 por de an antibody working for WB and IF This antibody recognizes a band of predicted molecular weight (1:500 dilution, 30 ug HeLa total extract), although the signal intensity is somewhat low. It also appears to work for IF (1:200 dilution, formaldehyde-fixed cells). One would expect a higher fluorescent signal if the antibody is diluted less.
Fecha de publicación: 2017-08-24
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