Rad51 Anticuerpo (F-11) recomendado para detectarRad51 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

Rad51 Anticuerpo (F-11): sc-398587

Rad51 Anticuerpo (F-11) clasificado 3.2 de 5 por 19.
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Ficha técnica

    • Anti-Rad51 Anticuerpo F-11 es un monoclonal de ratón IgG1 κ Rad51 Anticuerpo, ver las 23 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 311-339 en el C-terminus de Rad51 de origen human
    • Rad51 Anticuerpo (F-11) es recomendado para detectar Rad51 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • Rad51 Anticuerpo (F-11) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Rad51 (F-11): sc-398587.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Rad51 Antibody (F-11) for WB and IHC(P) applications. These reagents are now offered in bundles with Rad51 Antibody (F-11) (see ordering information below).
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Hello, I am thinking of trying sc-398587 Rad51 F-11 for IF in human cancer cells but I see some bad reviews for IF (and WB). Do you have protocol recommendations (as far as pre-extracting or not etc)? Thank you.

Preguntado por: adkjfd
Thank you for your question. sc-398587 is a great antibody for Rad51. It is recommended and warrantied for IF on human, mouse, and rat samples. Here is a recent publication using it for IF on human samples (PMID: 29694959). Here is a link to the recommended protocol. We recommend a starting dilution of 1:50. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Tech Service
Fecha de publicación: 2019-08-30

If I want to test the specificity of RAD51, can I also have a sample of the blocking peptide sc-398587 P? Please let me know. Thanks.

Preguntado por: harias
Thank you for your question. We do not provide sample sizes of the blocking peptides.
Respondida por: Technical Support
Fecha de publicación: 2019-05-25

Do you have the one which can be used for the flow cytometry?

Preguntado por: ning25348
Thank you for your question. Unfortunately we do not offer an antibody that has been tested for Flow cytometry against Rad51, so they can not be recommended or warrantied for that use. Please contact Technical Service if you have any further questions.
Respondida por: Tech Service
Fecha de publicación: 2019-01-14

How can I get a trial sample for the monoclonal Ab Rad51 (F-11): sc-398587? Please let me know.

Preguntado por: harias
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Respondida por: Technical Support
Fecha de publicación: 2018-12-05

What is the recommended antigen retrieval method when doing IHC(P) with this antibody?

Preguntado por: AbPolly
Thank you for your question. We recommend using sodium citrate buffer, pH 6.0, and heat for antigen retrieval. The full recommended protocol can be found on our website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-01-30
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Rated 1 de 5 por de very very bad no band (different size band coming) and no IF staining. very bad
Fecha de publicación: 2018-09-05
Rated 1 de 5 por de Didn't work in my hands. Tried for western at 1:500 dilution and didn't detect any band in human neuroblastoma cancer cells. The discontinued works perfectly fine in the same lysates.
Fecha de publicación: 2018-06-26
Rated 1 de 5 por de Tried twice for WB, just got a smeary mess. Tried this as a sample, very disappointed. I firstly tried 1:100 5% milk in TBST, incubated for 1.5 hours at room temperature. I got nothing but smears on the blot. I then tried 1:500 incubation for 1.5 hours at room temperature followed by overnight incubation at 4 oC. This is the blot shown in he picture. Nothing at 37kDa and lots of messy smears, does not work at all. Loaded 30ug protein per well with llamaeli buffer. Normally works very well. GAPDH control (not shown) also worked well.
Fecha de publicación: 2018-01-25
Rated 5 de 5 por de 这个抗体和之前一样好用! 多年前买过santa cruz抗体,质量很好,物美价廉。这次试用的抗体一如既往的好用,以后会继续关注santa cruz。
Fecha de publicación: 2017-12-19
Rated 5 de 5 por de Good antibody I tried this antibody for Rad51. It is very good! The blot is very clean and clear. The cell line is U2OS. The middle lane is from cells infected by Rad51 shRNA.
Fecha de publicación: 2017-11-04
Rated 3 de 5 por de can't recognize any band in NPC cell lysates can't recognize any band in NPC cell lysates,1:250 in 5%TBST,4℃ overnight
Fecha de publicación: 2017-09-22
Rated 5 de 5 por de Great for WB Works great for WB at 1:1000 dilution in HeLa and 293T cells.
Fecha de publicación: 2017-09-06
Rated 1 de 5 por de It is not good for IF. Tried this antibody, but there was no signal at 1:50 diluted.
Fecha de publicación: 2017-08-02
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