Rad51 Anticuerpo (3C10) recomendado para detectarRad51 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

Rad51 Anticuerpo (3C10): sc-53428

Rad51 Anticuerpo (3C10) clasificado 3.8 de 5 por 14.
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Ficha técnica

    • Rad51 Anticuerpo (3C10) es un monoclonal de ratón IgG1 λ, ver las 17 publicaciones, proporcionado como 200 µg/ml
    • raised against full length recombinant human RAD51 protein
    • Rad51 Anticuerpo (3C10) es recomendado para detectar Rad51 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • Rad51 Anticuerpo (3C10) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Rad51 (3C10): sc-53428.
    • m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for Rad51 Antibody (3C10) for WB and IHC(P) applications. This reagent is now offered in a bundle with Rad51 Antibody (3C10) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Why are two bands obtained in the western blot? is it nonspecific?

Preguntado por: ISMS
Thank you for your question. These bands are not unspcific signals. Rad51 has four described isoforms of different molecular weights (37 kDa, 26 kDa, 31 kDa, and 36 kDa, respectively). When different isoforms are present in a given sample, the antibody will detect multiple specific bands.
Respondida por: Technical Support Europe
Fecha de publicación: 2020-10-08

Which is the epitope recognized by this antibody?

Preguntado por: UOGL
Thank you for your question. Rad51 Antibody (3C10): sc-53428 was raised against full length recombinant human RAD51 protein. So far, no epitope mapping has been performed for this antibody, so its exact epitope is not yet known.
Respondida por: Tech Support Europe
Fecha de publicación: 2019-05-09

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Rad51 Antibody (3C10): sc-53428?

Preguntado por: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Respondida por: Technical Support 15
Fecha de publicación: 2017-01-24
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Rated 5 de 5 por de Great for WB in the mouse samples I got the free sample and tested in WB using lysates from mouse brains and mouse cell line N2A. The antibody gives strong signals with generally clean background. and I have ordered this antibody for the future use. Generally, I loaded 15 μg protein for each sample and used 1:1000 antibody dilution in 5% BSA/TBST for the membrane incubation. For the signal detection, I used pico PLUS ECL from Thermo fisher to develop the signals and detected using X-ray films.
Fecha de publicación: 2021-06-13
Rated 5 de 5 por de Works for western blot Used for western blot on ovcar3 cells. Able to get detection at 1:500 in BSA
Fecha de publicación: 2021-03-26
Rated 3 de 5 por de Segnale discreto Ho provato questo anticorpo su vescicole extracellulari e loro cellula sorgente. Ho rilevato il segnale nelle cellule, ma solo con ECL ultra.
Fecha de publicación: 2021-01-25
Rated 1 de 5 por de not suitable for IF and WB we used this antibody in different concentrations for IF (dilution 1:50 - 1:500, cells were fixed with either formaldehyde or methanol) and WB (dilution range 1:50 - 1:1000 in BSA/TBS-T or MMP/TBS-T) but recieved no usable signals
Fecha de publicación: 2020-01-09
Rated 1 de 5 por de Not good for IF in my hands The antibody was used for immunofluorescence analysis on murine paraformaldehyde-fixed ovarian cells, but there was no signal at 1:100 and 1:50 dilution.
Fecha de publicación: 2019-10-20
Rated 3 de 5 por de General product When western blot is using this antibody, the antibody has a bad specificity and a mixed band
Fecha de publicación: 2017-12-18
Rated 3 de 5 por de WB in T98G cells It is ok this antibody. It shows non specific bands but still you can guess which band is the one you are looking for just looking at the molecular weight. I used this antibody in T98G cells lysis, dilution of the antibody 1:200 in 5% milk-PBSTween incubating over night
Fecha de publicación: 2017-12-11
Rated 5 de 5 por de Good western blot results in human Good western blot results in human.
Fecha de publicación: 2015-03-02
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