Rad50 Anticuerpo (G-2) recomendado para detectarRad50 de mouse, rat y human origen, mediante WB, IP, IF y ELISA

Rad50 Anticuerpo (G-2): sc-74460

Rad50 Anticuerpo (G-2) clasificado 4.7 de 5 por 15.
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Ficha técnica

    • Anti-Rad50 Anticuerpo G-2 es un monoclonal de ratón IgG2a κ Rad50 Anticuerpo, ver las 13 publicaciones, proporcionado como 200 µg/ml
    • raised against amino acids 1013-1312 mapping at the C-terminus of Rad50 of human origin
    • Rad50 Anticuerpo (G-2) es recomendado para detectar Rad50 de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • Rad50 Anticuerpo (G-2) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Rad50 (G-2): sc-74460.
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for Rad50 Antibody (G-2) for WB applications. These reagents are now offered in bundles with Rad50 Antibody (G-2) (see ordering information below).
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What is the recommended fixation method for immunofluorescence staining with Rad50 (G-2): sc-74460 monoclonal antibody?

Preguntado por: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 4 de 5 por de OK for western blot OK for western blot (1:1000 dilution) in lung cancer cell lines
Fecha de publicación: 2018-07-19
Rated 5 de 5 por de Good for WB I used this antibody for my WB on MDA-MB231 cells. It gives me clean bands with a non specific bands below but no background.
Fecha de publicación: 2018-02-01
Rated 5 de 5 por de Great antibody Nice and strong by wb detection in MCF7 cell lines
Fecha de publicación: 2017-11-29
Rated 5 de 5 por de Good Worked for our rat cell line. Used at 1:1000 for WB. Good signal.
Fecha de publicación: 2017-11-15
Rated 5 de 5 por de Nice results by WB on mouse colon extracts We were able to detect the band at the proper MW on mouse colon extracts challenged by DNA damage. We used regular ECL and a 1/500 dilution for the ab. Saturation of the membrane and incubation with the ab was performed in TBS-Tween-5% non fat milk. The signal showed up after around 10 min of revelation
Fecha de publicación: 2017-10-27
Rated 5 de 5 por de Good one The sample antibody worked well for WB in HEK-293T cells at 1:500 dilution.
Fecha de publicación: 2017-08-16
Rated 1 de 5 por de This antiody did not work at 1:1000 in WB of U2OS This antibody did not work at 1:1000 in WB of U2OS cells.
Fecha de publicación: 2017-07-05
Rated 5 de 5 por de High Quality Product The Rad50 Antibody (G-2): sc-74460 provides excellent data for immunofluorescence applications showing staining pattern with nuclear localization. This antibody has also been cited multiple times for further verification.
Fecha de publicación: 2017-02-03
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