Phosphatase Inhibitor Cocktail A Inhibidor de la L-isozima de la fosfatasa alcalina y proteínas fosfatasas Ser / Thr

Phosphatase Inhibitor Cocktail A

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Nombres Alternativos: Phosphatase Inhibitor Cocktail I
Solicitud: Phosphatase Inhibitor Cocktail A is an inhibitor of L-isozymes of alkaline phosphatase and ser/thr protein phosphatases
Para Uso Exclusivo en Investigación. No está diseñado para uso en diagnosis o terapia.
* En el Certificado de Análisis específico de lote, puede encontrar información específica (como el contenido en agua).
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Phosphatase Inhibitor Cocktail A effectively inhibits L-isozymes of alkaline phosphatase and serine/threonine protein phosphatases, such as the PP1 (protein phosphatase 1) and PP2A) protein phosphatase 2a) proteins. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Recommended for use with tissue and cell extracts, including extracts containing detergents.
Each vial contains 2.5 mM (-)-p-Bromotetramisole oxalate, 500 µM Cantharidin and 500 nM Microcystin-LR in 1 ml DMSO. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


References

1. Nishiwaki, S., et al. 1991. FEBS Lett. 279: 115-118. PMID: 1847341
2. Ilkovitch, D., et al. 2008. Cancer Res. 68: 2427-2435. PMID: 18381451
3. Wagner, M.W., et al. 2008. J. Biol. Chem. 283: 21382-21393. PMID: 18480061
4. Jain, M.K. 1982. Handbook of Enzyme Inhibitors. New York: John Wiley and Sons, Pg. 222, 334.

Uso :
• Recommended usage is 100-fold dilution.

• One ml of Phosphatase Inhibitor Cocktail A will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, cow liver, rabbit muscle, A431 or Jurkat cell extracts at a protein concentration of approximately 5 mg/ml.

• One ml of Phosphatase Inhibitor Cocktail A solution is used to prepare 100 ml of supernatant that contains a maximum of 500 mg of protein. Therefore, 1 ml of cocktail should be added per 500 mg of protein extracted from the tissue used, or 1 ml of cocktail solution per 100 ml of extraction buffer.
Estado de Materia :
Liquid
ALMACENAMIENTO :
Store at -20° C
Para Uso Exclusivo en Investigación. No está diseñado para uso en diagnosis o terapia.

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CERTIFICADO DE ANÁLISIS

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Phosphatase Inhibitor Cocktail A  Menciones del Producto

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Citaciones 1 a 10 de un total de 54

PMID: # 33500244  Duy, C.|Li, M.|Teater, M.|Meydan, C.|Garrett-Bakelman, FE.|Lee, TC.|Chin, CR.|Durmaz, C.|Kawabata, KC.|Dhimolea, E.|Mitsiades, CS.|Doehner, H.|D'Andrea, RJ.|Becker, MW.|Paietta, EM.|Mason, CE.|Carroll, M.|Melnick, AM.| et al. 2021. Cancer Discov.

PMID: # 33441552  Wu, CL.|Dicks, A.|Steward, N.|Tang, R.|Katz, DB.|Choi, YR.|Guilak, F.| et al. 2021. Nat Commun. 12: 362.

PMID: # 31606082  Tiambeng, TN.|Tucholski, T.|Wu, Z.|Zhu, Y.|Mitchell, SD.|Roberts, DS.|Jin, Y.|Ge, Y.| et al. 2019. Methods Enzymol. 626: 347-374.

PMID: # 30942458  Ibrahim, SM.|Bakhashab, S.|Ilyas, AM.|Pushparaj, PN.|Karim, S.|Khan, JA.|Abuzenadah, AM.|Chaudhary, AG.|Al-Qahtani, MH.|Ahmed, F.| et al. 2019. Oncol. Rep. 41: 3179-3188.

PMID: # 30817799  Grant, MKO.|Handoko, M.|Rozga, M.|Brinkmalm, G.|Portelius, E.|Blennow, K.|Ashe, KH.|Zahs, KR.|Liu, P.| et al. 2019. PLoS ONE. 14: e0212815.

PMID: # 30979869  Yang, Y.|van der Klaauw, AA.|Zhu, L.|Cacciottolo, TM.|He, Y.|Stadler, LKJ.|Wang, C.|Xu, P.|Saito, K.|Hinton, A.|Yan, X.|Keogh, JM.|Henning, E.|Banton, MC.|Hendricks, AE.|Bochukova, EG.|Mistry, V.|Lawler, KL.|Liao, L.|Xu, J.|O'Rahilly, S.|Tong, Q.|, .|Inês Barroso, .|O'Malley, BW.|Farooqi, IS.|Xu, Y.| et al. 2019. Nat Commun. 10: 1718.

PMID: # 29491408  Shi, Q.|Viswanadhapalli, S.|Friedrichs, WE.|Velagapudi, C.|Szyndralewiez, C.|Bansal, S.|Bhat, MA.|Choudhury, GG.|Abboud, HE.| et al. 2018. Sci Rep. 8: 3781.

PMID: # 30050412  Shi, Q.|Saifetiarova, J.|Taylor, AM.|Bhat, MA.| et al. 2018. Front Cell Neurosci. 12: 201.

PMID: # 30148884  Ho Wei, L.|Arastoo, M.|Georgiou, I.|Manning, DR.|Riobo-Del Galdo, NA.| et al. 2018. PLoS ONE. 13: e0203170.

PMID: # 28487416  Amar, F. et al. 2017. Sci Signal. 10:

Citaciones 1 a 10 de un total de 54
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