PCLN-1 Antibody (7A2): sc-130561

PCLN-1 Antibody (7A2) is a mouse monoclonal IgM antibody that detects PCLN-1 of mouse, rat, and human origin by immunofluorescence (IF) and enzyme-linked immunosorbent assay (ELISA). Anti-PCLN-1 antibody (7A2) is available as the non-conjugated format. PCLN-1, also known as paracellin-1, plays a crucial role in forming and maintaining tight junctions, which regulate the paracellular pathway between epithelial and endothelial cells. These tight junctions create a barrier preventing free passage of solutes and ions, maintaining cellular homeostasis and tissue integrity. PCLN-1 localizes to kidney tissue tight junctions, forming intercellular pores vital for magnesium and calcium resorption in the thick ascending limb of Henle. PCLN-1′s structure includes four transmembrane domains and intracellular amino and carboxy termini, characteristic of the claudin family. Mutations in PCLN-1 can lead to renal magnesium wasting, contributing to a rare autosomal recessive disorder known as renal hypomagnesemia with hypercalciuria and nephrocalcinosis, highlighting PCLN-1′s significance in renal function and electrolyte balance.

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PCLN-1 Antibody (7A2) References:

1. Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption.  |  Simon, DB., et al. 1999. Science. 285: 103-6. PMID: 10390358
2. Paracellular channels!  |  Wong, V. and Goodenough, DA. 1999. Science. 285: 62. PMID: 10428705
3. Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions.  |  Kubota, K., et al. 1999. Curr Biol. 9: 1035-8. PMID: 10508613
4. Manner of interaction of heterogeneous claudin species within and between tight junction strands.  |  Furuse, M., et al. 1999. J Cell Biol. 147: 891-903. PMID: 10562289
5. Tight junctions and the molecular basis for regulation of paracellular permeability.  |  Anderson, JM. and Van Itallie, CM. 1995. Am J Physiol. 269: G467-75. PMID: 7485497
6. Renal magnesium handling and its hormonal control.  |  de Rouffignac, C. and Quamme, G. 1994. Physiol Rev. 74: 305-22. PMID: 8171116
7. Hypomagnesemia: renal magnesium handling.  |  Kelepouris, E. and Agus, ZS. 1998. Semin Nephrol. 18: 58-73. PMID: 9459289
8. Regulation of the movement of solutes across tight junctions.  |  Madara, JL. 1998. Annu Rev Physiol. 60: 143-59. PMID: 9558458
9. Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands.  |  Morita, K., et al. 1999. Proc Natl Acad Sci U S A. 96: 511-6. PMID: 9892664

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PCLN-1 Antibody (7A2) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PCLN-1 Antibody (7A2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PCLN-1 Antibody (7A2) Specifications

Alternate Product Name: PCLN-1

Product Clone Name: 7A2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: PCLN-1

Epitope Species: human

Product Reactivity/Detection: PCLN-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PCLN-1 Antibody (7A2): N/A