p-Tyr Anticuerpo (PY99) recomendado para detectar phosphotyrosine-containing proteins medainte WB, IP, IF y IHC(P)

p-Tyr Anticuerpo (PY99): sc-7020

p-Tyr Anticuerpo (PY99) clasificado 4.7 de 5 por 31.
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Ficha técnica

    • p-Tyr Anticuerpo (PY99) es un monoclonal de ratón IgG2b κ, ver las 2.640 publicaciones, proporcionado como 200 µg/ml
    • raised against p-Tyr
    • p-Tyr Anticuerpo (PY99) es recomendado para detectar phosphotyrosine-containing proteins medainte WB, IP, IF y IHC(P); no tiene reactividad cruzada con phosphoserine or phosphothreonine
    • p-Tyr Anticuerpo (PY99) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • disponible conjugado a biotina para WB, IHC(P) y ELISA; y a Alexa Fluor® 405 para IF, IHC(P) y FCM
    • también disponibles conjugados a Alexa Fluor® 405 para IF, IHC(P) y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de p-Tyr (PY99): sc-7020.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Tyr Antibody (PY99). This work is in progress.
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Is there anybody know the difference between PY20 and PY99?

Preguntado por: shaw
Thank you for your question. p-Tyr Antibody (PY99) is recommended for detection of phosphotyrosine-containing proteins by WB, IP, IF and IHC(P); non cross-reactive with phosphoserine or phosphothreonine. p-Tyr Antibody (PY20) is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM.
Respondida por: Technical Support 12
Fecha de publicación: 2021-10-12

Dear Santa Cruze Do i have to boil/unboil my cell lysate before i use this p-Tyr(PY99) for western blot application?

Preguntado por: Kenny C
Thank you for your question. Yes, you should boil your lysate before loading it onto your gel.
Respondida por: Tech Service
Fecha de publicación: 2019-11-25

When I use this antibody for immunohistochemistry. Is antigen retrieval necessary?

Preguntado por: AH2019
Thank you for your question. Yes, we recommend performing an antigen retrieval step if you are doing IHC on formalin fixed paraffin embedded tissue sections.
Respondida por: Tech Service
Fecha de publicación: 2019-10-15

Can I use 10% milk to block when using this antibody? I am interested in getting it conjugated with the alexa 790 to be used with LI-COR®Odyssey®.

Preguntado por: nsal
Thank you for your question. We recommend blocking in 5% milk with TBS-T, and it should be fine with the LiCOR Odyssey.
Respondida por: Tech Service
Fecha de publicación: 2019-09-24

Is there any species specificity for this antibody? Or is there any cross-reactivity?

Preguntado por: beni
Thank you for your question. This antibody has broad species reactivity and is specific for phosphotyrosine-containing proteins; non cross-reactive with phosphoserine or phosphothreonine.
Respondida por: Technical Support
Fecha de publicación: 2019-09-11

I want to measure insulin sensitivity and several tyrosine domains are phosphorylated in response to insulin. For example in both insulin receptors and in IRS. Does the antibody detetct all p-tyr, and I differentiate between them depending on weight?

Preguntado por: Ahmed_hassan
Thank you for your question. Yes, you are correct, this mouse monoclonal antibody, p-Tyr (PY99): sc-7020, specifically detects phosphotyrosine-containing proteins (and does not cross-reactive with phosphoserine or phosphothreonine-containing proteins). If you are using the antibody for Western blotting, then the molecular weight you detect in your samples is representative of different phosphorylated proteins. You would need to do further testing to identify which specific proteins are phosphorylated in your samples.
Respondida por: Technical Support
Fecha de publicación: 2021-04-06

What secondary reagent should I use with p-Tyr Antibody (PY99) for Western blot?

Preguntado por: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Tech Service 8
Fecha de publicación: 2016-12-15

Is it possible to use this p-Tyr (PY99) mouse monoclonal antibody for immunohistochemistry in mouse tissue?

Preguntado por: Trav11
Yes, it is possible. If working with mouse tissue, we recommend using our directly conjugated mouse monoclonal primary antibodies. The p-Tyr Antibody (PY99): sc-7020 is currently available conjugated to phycoerythrin (sc-7020 PE), fluorescein (sc-7020 FITC), Alexa Fluor® 488 (sc-7020 AF488), Alexa Fluor® 647 (sc-7020 AF647), Alexa Fluor® 405 (sc-7020 AF405), biotin (sc-7020 B) or alkaline phosphatase (sc-7020 AP).
Respondida por: Tech Service 8
Fecha de publicación: 2019-11-07
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Rated 3 de 5 por de Pretty good antibody The antibody works pretty well upon IP whereas on total cellular extract is too weak.
Fecha de publicación: 2020-10-07
Rated 5 de 5 por de detection of phosphotyrosine-containing proteins I recommend for detection of phosphotyrosine-containing proteins!
Fecha de publicación: 2019-12-04
Rated 4 de 5 por de good pTr antibody Works well to detect tyrosine phosphorylation in whole cell or tissue lysates.
Fecha de publicación: 2018-05-17
Rated 5 de 5 por de good for WB I use 1:1000 in 5%BSA, it is less specific for mouse lung tissue, I haven't try primary cell but I hope it works.
Fecha de publicación: 2018-03-12
Rated 4 de 5 por de Good for WB I tried a 1/500 dilution in 3% BSA and it worked well. Good signal.
Fecha de publicación: 2017-10-24
Rated 5 de 5 por de IHC detection IHC detected the expression of P-Tyr in gastric carcinoma tissues ,it gave cytoplasmic staining ,good signal for us.
Fecha de publicación: 2017-09-16
Rated 5 de 5 por de staining IHC(1/100) staining in human skin tissu, very sensitive
Fecha de publicación: 2017-09-08
Rated 5 de 5 por de very good Gives clear for nuclear extracts in western blot; had a strong signal in the western blot.
Fecha de publicación: 2017-08-17
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