p-Tyr Anticuerpo (PY20) recomendado para detectar broad species mediante WB, IP, IF, IHC(P) y FCM

p-Tyr Anticuerpo (PY20): sc-508

p-Tyr Anticuerpo (PY20) clasificado 4.8 de 5 por 19.
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Ficha técnica

    • p-Tyr Anticuerpo (PY20) es un monoclonal de ratón IgG2b κ, ver las 1.306 publicaciones, proporcionado como 200 µg/ml
    • raised against p-Tyr
    • p-Tyr Anticuerpo (PY20) es recomendado para detectar broad species mediante WB, IP, IF, IHC(P) y FCM
    • p-Tyr Anticuerpo (PY20) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • también disponibles conjugados a Alexa Fluor® 405 para IF, IHC(P) y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de p-Tyr (PY20): sc-508.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p-Tyr Antibody (PY20) for WB and IHC(P) applications. These reagents are now offered in bundles with p-Tyr Antibody (PY20) (see ordering information below).
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Hello! Please tell me if p-Tyr Antibody (PY20): sc-508 is working in ELISA assay. If yes, could you recommend me a starting dilution range! I want to know if the p-Tyr Antibody (PY20) HRP variant is recommended for ELISA and in what dilution.

Preguntado por: Rodica
Thank you for your question. p-Tyr Antibody (PY20): sc-508 is recommended for detection of broad species by WB, IP, IF, IHC(P) and FCM. It is also available conjugated to HRP for ELISA. The starting dilution is 1:30, with a dilution range of 1:30-1:3000.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-11-27

Which phosphotyrosine antibody is more sensitive in Western Blot: p-Tyr (PY99) or p-Tyr (PY20)? Which concentration is the recommended?

Preguntado por: AngelesMZ
Thank you for your question. Both p-Tyr Antibody (PY20): sc-508 and p-Tyr Antibody (PY99): sc-7020 are featured in several hundred publications and are recommended for western blot and other applications. Both antibodies have a recommended starting dilution of 1:200 and work in a dilution range 1:100-1:1000. Either antibody is perfectly suited for western blotting, so you could order one or the other or get in touch with our technical service to arrange for free samples of the two antibodies.
Respondida por: Technical Support Europe
Fecha de publicación: 2019-04-12

for blocking, what would you recommend? Many people recommend 5% of BSA for phospho-protein..( should I stick with this rule.). but some phospho-protein can worked well with.. Skim milk (depends the antibody protocol..)

Preguntado por: blessing
Thank you for your question. We recommend using 1% milk, 1% BSA in 0.05% Tween-20 for blocking and antibody incubations.
Respondida por: Technical Support
Fecha de publicación: 2019-02-01

EGF-treated とは、具体的にどのようにするものでしょうか。ご教授お願い致します。

Preguntado por: k 2018
Thank you for your question. Please contact our Technical Service Department and we will be happy to assist you. You may call us directly at 001 800-1338-3838, email at [email protected], or contact us by live chat. お問い合わせを頂きまして、誠にありがとうございました。お手数ですが、電話 001 800-1338-3838、メール[email protected]またはライブチャットで、当社のテクニカルサービスまでお問い合わせください。その他の不明点等がございましたら、またご連絡ください。
Respondida por: Technical Support 8
Fecha de publicación: 2018-12-21

Can I use p-Tyr Antibody (PY20): sc-508 for immunohistochemical staining of mouse tissue

Preguntado por: Anónimo
We recommend using a directly conjugated mouse monoclonal primary antibody. See p-Tyr (PY99): sc-7020 for p-Tyr antibody conjugates, including HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. https://www.scbt.com/scbt/product/p-tyr-antibody-py99
Respondida por: Tech Service 6
Fecha de publicación: 2016-12-16

What is the recommended secondary detection reagent in Western blot?

Preguntado por: Anónimo
We recommend our Mouse IgG Binding Protein conjugated to HRP as a secondary detection reagent: https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Tech Service 6
Fecha de publicación: 2016-12-16
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Rated 5 de 5 por de Always reliable! I have relied on you for over 20 years and you have always delivered an excellent product on time. Your phone staff is friendly and helpful.
Fecha de publicación: 2018-03-08
Rated 4 de 5 por de Very good I have used Py20 Antibody from Santa and it worked great.
Fecha de publicación: 2017-12-14
Rated 4 de 5 por de As expected Gives WB bands as expected at 1:500 dilution in 5% SA (block in non-fat dry milk).
Fecha de publicación: 2017-12-02
Rated 5 de 5 por de Very Strong Signal I compare different products for art antibody found p-Tyr antibody is the most sensitive, sc-508 is the most reliable quality.
Fecha de publicación: 2017-10-28
Rated 5 de 5 por de Excellent antibody Strong signal and low background on immunoprecipitated protein.
Fecha de publicación: 2017-09-01
Rated 5 de 5 por de good result antibody It is an excellent antibody. gives a single band in my hand.
Fecha de publicación: 2017-05-27
Rated 5 de 5 por de antibody for WB I use the dilution 1:800, get very clean bands.I like
Fecha de publicación: 2017-05-07
Rated 5 de 5 por de High Quality Great antibody for detection of any protein with a phosphorylated Tyrosine, can be used in a number of applications, not species specific.
Fecha de publicación: 2016-12-20
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