p-Ser/Phosphoserine Anticuerpo (16B4) recomendado para detectara broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine de mouse, rat, human y canine origen, mediante WB y IP

p-Ser/Phosphoserine Anticuerpo (16B4): sc-81514

p-Ser/Phosphoserine Anticuerpo (16B4) clasificado 3.9 de 5 por 22.
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Ficha técnica

    • Anti-p-Ser/Phosphoserine Anticuerpo 16B4 es un monoclonal de ratón IgM κ p-Ser/Phosphoserine Anticuerpo, ver las 64 publicaciones, proporcionado como 50 µg/0.5 ml
    • raised against phosphoserine
    • recomendado para detectara broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine de mouse, rat, human y canine origen, mediante WB y IP
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de p-Ser/Phosphoserine (16B4): sc-81514.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for p-Ser/Phosphoserine Antibody (16B4). This work is in progress.
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Is there any possibility that p-Ser Antibody (16B4): sc-81514 does work for yeast samples as well...?

Preguntado por: yolyol
Thank you for your question. This antibody has only been tested in samples of mouse, rat, human and canine origin. It has not been tested in yeast species.
Respondida por: Tech Support Europe
Fecha de publicación: 2021-02-26

Hello, Which buffer is better to detect p-Ser antibody, skim-milk or 3-5% BSA? I immunoprecipitated phospho-protein and used 3% BSA in TBS-T buffer for blocking and 1st antibody but I couldn't get specific phosphorylation band.

Preguntado por: chohee0720
Thank you for your question. Please contact Technical Service directly for troubleshooting advice on your experiment.
Respondida por: Tech Support Europe
Fecha de publicación: 2021-05-11

HI , I recently purchased this pserine antibody and I do see a band at 20kDa. why do i see a band arounf 20kda for pSerine?

Preguntado por: Dhanusha
Thank you for your question. As p-Ser Antibody (16B4): sc-81514 detects a broad range of serine-phosphorylated proteins in crude cell extracts, a signal at 20 kDa can respond to a large variety of different proteins of this molecular weight. The identity of this protein could be determined by assays such as MALDI-TOF.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-07-14

The p-Ser Antibody (16B4) antibody is a monoclonal mouse IgM κ (kappa light chain). Still, you recommend the m-IgGκ BP-HRP: sc-516102 as a secondary antibody. But this a IgG-kappa specific ab. Would it still work?

Preguntado por: PeterPM
Thank you for your question. Yes, m-IgGκ BP-HRP: sc-516102 can detect any antibody that possesses a kappa light chain, including p-Ser Antibody (16B4): sc-81514, other IgM, and IgA antibodies.
Respondida por: Technical Support Europe
Fecha de publicación: 2019-05-06

Just wondering if your products of sc-81516 and sc-81514 recognize any of the phosphorylated S/T/Y, or they require certain residues beside these phosphorylated residues (like a certain motif).

Preguntado por: Zhyuhao
Thank you for your question. These antibodies both detect a broad range of serine-phosphorylated proteins in crude cell extracts, preferring positively charged amino acids directly neighbored to phosphoserine. Additional information regarding the detected residues is not available.
Respondida por: Tech Service
Fecha de publicación: 2019-04-10

"preferring positively charged amino acids directly neighbored to phosphoserine". Does it mean that phosphoserine without neighboring positively charged amino acids will not be recognized by the 16B4 Ab ?

Preguntado por: Yo14
Thank you for your question. The antibody recognizes a broad range of serine-phosphorylated proteins in crude cell extracts, interacting with pSK and pSP motifs strongly, recognizing substrates of MAP/SAP kinases and CDC kinases. Phosphoserine detection using an antibody generally is affected by the surrounding amino acids sequence, but it is possible that it can detect phosphoserine without neighboring + charged amino acids.
Respondida por: Tech Service
Fecha de publicación: 2018-08-09

Hi, is there any possibility to receive a sample from this antibody? Thanks for your answer.

Preguntado por: Constanze
Thank you for your question. We do not currently offer sample sizes for our p-Ser Antibody (16B4): sc-81514. This is a very popular and well cited antibody. If you purchase the antibody, please know that it is covered by our generous product warranty for one year from the date of purchase. If it does not work in any of the recommended applications, please contact our Technical Service Department and we can provide you with a replacement or refund.
Respondida por: Technical Support
Fecha de publicación: 2017-03-01

What is the expected molecular weight in Western blot when using p-Ser Antibody (16B4): sc-81514?

Preguntado por: cjMara
Thank you for your question. This antibody is recommended for detection of a broad range of serine-phosphorylated proteins. Therefore, the observed molecular weight is dependent upon which specific phosphorylated protein(s) you are evaluating with this antibody.
Respondida por: Technical Support
Fecha de publicación: 2016-12-22
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Rated 1 de 5 por de 使えなかった 一切バンドがでなかった。通常リン酸化抗体はブロードなバンドがみえるが、ひとつもバンドがみえなかった。
Fecha de publicación: 2020-11-18
Rated 3 de 5 por de Pretty good antibody The antibody works pretty well upon immunoprecipitation of the phosphorylated protein. On total cellular extract is too weak.
Fecha de publicación: 2020-10-07
Rated 3 de 5 por de Worked in WB phosphorylation was detected in Pull downed protein in WB
Fecha de publicación: 2019-07-23
Rated 5 de 5 por de Successful detection Successful detection of Ser phosphorylation in immunoprecipitates
Fecha de publicación: 2018-12-07
Rated 3 de 5 por de P-Ser Antibody Compared to other p-ser abs there was a lower background. Signal was much lower too. Tested in HeLa total lysates.
Fecha de publicación: 2018-02-14
Rated 3 de 5 por de Nice antibody We used this antibody for IP last month. It works well for some primary proteins. Overall a decent buy.
Fecha de publicación: 2017-11-13
Rated 4 de 5 por de works well I have tried this antibody in cells deprived for glucose. The results were good and clean
Fecha de publicación: 2017-10-27
Rated 5 de 5 por de Great results The results are quit stable. Exposure a little bit longer, you will get very clear ban.
Fecha de publicación: 2017-10-03
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