N-Myc Anticuerpo (3C165) recomendado para detectarN-Myc p67 de mouse, rat y human origen, mediante WB, IP y IF

N-Myc Anticuerpo (3C165): sc-71633

N-Myc Anticuerpo (3C165) clasificado 4.0 de 5 por 1.
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Ficha técnica

    • N-Myc Anticuerpo 3C165 es un monoclonal de ratón IgG2a, ver las 1 publicaciones, proporcionado como 200 µg/ml
    • epitope mapping at residues 327-339 of N-Myc of human origin
    • recomendado para detectarN-Myc p67 de mouse, rat y human origen, mediante WB, IP y IF
    • Ver N-Myc (B8.4.B): sc-53993 para N-Myc anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for N-Myc Antibody (3C165). This work is in progress.
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How much sample do you recommend loading on the gel for Western blot detection using N-Myc (3C165): sc-71633?

Preguntado por: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-03-01
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Rated 4 de 5 por de WB: Good results with human medulloblastoma cells WB: Good results with human medulloblastoma cells. -SCBT Publication Review
Fecha de publicación: 2010-03-01
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