Anti-Myosin Vb Anticuerpo 18 es un monoclonal de ratón IgG1 κ Myosin Vb Anticuerpo, ver las 1 publicaciones, proporcionado como 200 µg/ml
raised against a recombinant protein mapping within amino acids 940-1051 of Myosin Vb of rat origin
recomendado para detectarMyosin Vb de mouse y rat origen, mediante WB y IP
Contacte con nosotros para recibir GRATIS 10 µg de muestra de Myosin Vb (18): sc-135995.
m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) is the preferred secondary detection reagent for Myosin Vb Antibody (18) for WB applications. This reagent is now offered in a bundle with Myosin Vb Antibody (18) (see ordering information below).
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Myosin Vb Antibody (18) is a high quality monoclonal Myosin Vb antibody (also designated Myosin Vb antibody) suitable for the detection of the Myosin Vb protein of mouse and rat origin. Myosin Vb Antibody (18) is available as the non-conjugated anti-Myosin Vb antibody. Class V unconventional Myosins, which include Myosin Va and Myosin Vb, are nonfilamentous, Actin-binding enzymes that appear to be expressed ubiquitously. Myosin V proteins are regulated by their heavy chain phosphorylation, which occurs at the C-terminal tail domain. Myosin Vb, also known as MYO5B, is a 1,849 amino acid protein that may be involved in intracellular trafficking. Considered a Rab 8A interacting protein, Myosin Vb regulates intracellular trafficking of GluR from recycling endosomes (REs) to synaptic sites during long-term potentiation. Association with REs triggers rapid spine recruitment of endosomes and local exocytosis in spines. It is suggested that Myosin Vb is required for Insulin-induced Glut4 translocation in muscle cells.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using Myosin Vb (18): sc-135995 antibody for immunoprecipitation, how much antibody should I use?
Preguntado por: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here:
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