MYL9/MYL12A/B Anticuerpo (E-4) recomendado para detectar the myosin regulatory light chains encoded by MYL9, MYL12A and MYL12B, and LOC391722 of human origin; Mylc2b, Myl9 and Myl12a of mouse origin; and Mrlcb and Myl9 of rat origin medainte WB, IP, IF, IHC(P) y ELISA

MYL9/MYL12A/B Anticuerpo (E-4): sc-28329

MYL9/MYL12A/B Anticuerpo (E-4) clasificado 4.5 de 5 por 10.
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Ficha técnica

    • Anti-MYL9/MYL12A/B Anticuerpo E-4 es un monoclonal de ratón IgG1 κ MYL9/MYL12A/B Anticuerpo, ver las 25 publicaciones, proporcionado como 200 µg/ml
    • raised against amino acids 1-172 representing full length MRCL3 of human origin
    • MYL9/MYL12A/B Anticuerpo (E-4) es recomendado para detectar the myosin regulatory light chains encoded by MYL9, MYL12A and MYL12B, and LOC391722 of human origin; Mylc2b, Myl9 and Myl12a of mouse origin; and Mrlcb and Myl9 of rat origin medainte WB, IP, IF, IHC(P) y ELISA
    • MYL9/MYL12A/B Anticuerpo (E-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de MYL9/MYL12A/B (E-4): sc-28329.
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for MYL9/MYL12A/B Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with MYL9/MYL12A/B Antibody (E-4) (see ordering information below).

I am looking for an AB that will stain phosphorylated LC-20. Can this AB be used for this purpose or do you provide another version of AB that stains for phospho-LC-20 as well?

Preguntado por: doksana
Thank you for your question. MYL9/MYL12A/B Antibody (E-4): sc-28329 will recognize its target protein in both phosphorylated and unphosphorylated form. The antibody is unable to distinguish between the two forms however.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-07-29

Could I use this MYL9/MYL12A/B Antibody (E-4): sc-28329 antibody for immunofluorescence of human bone marrow-derived mesenchymal stem cells(hBMSCs)? Is it only for smooth muscle myosins, or also for non-muscle myosins? Thanks.

Preguntado por: taiyang
Thank you for your question. MYL9/MYL12A/B Antibody (E-4): sc-28329 has been raised against amino acids 1-172 representing full length MRCL3 of human origin. It is recommended for recommended for detection of the myosin regulatory light chains encoded by MYL9, MYL12A and MYL12B, and LOC391722 of human origin; Mylc2b, Myl9 and Myl12a of mouse origin; and Mrlcb and Myl9 of rat origin by immunofluorescence, WB, IP, IHC(P) and ELISA. For further information, please contact our technical service directly.
Respondida por: SCBT Heidelberg Support
Fecha de publicación: 2019-01-06

Can I use MYL9/MYL12A/B (E-4): sc-28329 mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Preguntado por: cjMara
Yes. We recommend either using a directly conjugated monoclonal antibody. MYL9/MYL12A/B (E-4): sc-28329 monoclonal antibody is available directly conjugated to either HRP, FITC, PE, Alexa Fluor® 488 or Alexa Fluor® 647. Alternatively, we recommend performing indirect staining using our recommended mouse IgG binding protein conjugated to HRP (sc-516102). https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp?requestFrom=search
Respondida por: Technical Support 5
Fecha de publicación: 2017-01-20
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Rated 1 de 5 por de The Myl9(E4) didn't work The Myl9(E4) didn't work in C2C12, HEK293, MDCK and Rat2 cell lysates. I used 1:1000 dilution.
Fecha de publicación: 2017-12-04
Rated 4 de 5 por de Buen resultado Nos mandaron la prueba del anticuerpo hace dos semanas. Lo usamos en western blot, en muestras invitro, con una concentración de 1:1000 y con 1% de BSA en TBST. Funcionó bastante bien, se veía una señal clara y no necesitaba mucha exposición. Compraremos el original próximamente.
Fecha de publicación: 2017-04-19
Rated 5 de 5 por de Just a great product Outstanding product for western blot, IF and IHC staining
Fecha de publicación: 2017-02-05
Rated 5 de 5 por de Clean WB Results! Great performance in Western, clean bands with no background. Also good in IF and IHC.
Fecha de publicación: 2016-12-23
Rated 5 de 5 por de Good antibody This MRLC antibody is good for Western blot experiment in C2C12 cells.
Fecha de publicación: 2016-11-02
Rated 5 de 5 por de Western blot publication using MCF Western blot publication using MCF-10A samples -SCBT Publication Review
Fecha de publicación: 2015-03-27
Rated 5 de 5 por de Mouse astrocytes published in WB application Mouse astrocytes published in WB application -SCBT Publication Review
Fecha de publicación: 2015-03-23
Rated 5 de 5 por de WB publication using rat VSMCs WB publication using rat VSMCs -SCBT Publication Review
Fecha de publicación: 2015-03-20
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