MYH7 Anticuerpo (A4.840) recomendado para detectarmyosin heavy chain 7 de mouse, rat, human, rabbit y avian origen, mediante WB, IP, IF y IHC(P)

MYH7 Anticuerpo (A4.840): sc-53089

MYH7 Anticuerpo (A4.840) clasificado 4.6 de 5 por 11.
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Ficha técnica

    • Anti-MYH7 Anticuerpo A4.840 es un monoclonal de ratón IgM κ MYH7 Anticuerpo, ver las 26 publicaciones, proporcionado como 200 µg/ml
    • raised against adult skeletal muscle myosin of human origin
    • recomendado para detectarmyosin heavy chain 7 de mouse, rat, human, rabbit y avian origen, mediante WB, IP, IF y IHC(P)
    • disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; ficoeritrina, fluoresceina, Alexa Fluor® 488 ó Alexa Fluor® 647 para IF, IHC(P) y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de MYH7 (A4.840): sc-53089.

Does this antibody work for rat tissue? Also, what is the recommended lysis buffer for homogenization?

Preguntado por: NotTom
Thank you for your inquiry! Yes, this antibody is mouse, rat and human reactive, so you can use it on your rat tissues. We recommend using RIPA buffer as a lysis buffer. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Respondida por: Tech Service
Fecha de publicación: 2017-01-17

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Preguntado por: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Respondida por: Technical Support 2
Fecha de publicación: 2017-01-11

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Preguntado por: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-53089 PE), fluorescein (sc-53089 FITC), Alexa Fluor® 488 (sc-53089 AF488) or Alexa Fluor® 647 (sc-53089 AF647). Please contact Technical Service if you have further questions concerning this product.
Respondida por: Technical Support 2
Fecha de publicación: 2017-01-11
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Rated 2 de 5 por de not working I tested for SD rat aortic aortic smooth muscle cells,Regrettably,I can not got any results,Probably,it is the myh7 molecular weight is so large
Fecha de publicación: 2017-07-13
Rated 4 de 5 por de It is not very good in C2C12 WB It is not as good as MYH B5 in WB for C2C12 differentiation for 7 days. 1:500 dilution
Fecha de publicación: 2017-05-31
Rated 5 de 5 por de MYH7 antibody I have tried the MYH7 antibody in Western blot, MYH7 antibody is very good, the band is approximately at 220kDa and seems so clear.
Fecha de publicación: 2017-05-15
Rated 5 de 5 por de Superb product Great data for all applications including WB,IF and IHC
Fecha de publicación: 2017-01-29
Rated 5 de 5 por de Good antibody for WB The MYH7 Antibody (A4.840): sc-53089 provides good data for Western blot, showing clear band with minimal background.
Fecha de publicación: 2017-01-13
Rated 5 de 5 por de Well done in WB using heart tissue extracts This antibody work well in WB using heart tissue extracts of mouse.
Fecha de publicación: 2016-11-16
Rated 5 de 5 por de Robust cytoplasmic staining in paraffin Robust cytoplasmic staining in paraffin-embedded human heart muscle. -SCBT QC
Fecha de publicación: 2015-03-22
Rated 5 de 5 por de IF: Very good nuclear and cytoplasmic staining IF: Very good nuclear and cytoplasmic staining in HeLa cells. -SCBT QC
Fecha de publicación: 2015-03-09
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