Anti-MYH Anticuerpo B-5 es un monoclonal de ratón IgG1 κ MYH Anticuerpo, ver las 40 publicaciones, proporcionado como 200 µg/ml
producido contra los amino ácidos 1641-1940 de myosin heavy chain 3 de origen human
MYH Anticuerpo (B-5) es recomendado para detectar skeletal and cardiac myosin heavy chain de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
MYH Anticuerpo (B-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de MYH (B-5): sc-376157.
m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for MYH Antibody (B-5) for WB and IHC(P) applications. These reagents are now offered in bundles with MYH Antibody (B-5) (see ordering information below).
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
MYH Antibody (B-5) is a high quality monoclonal MYH antibody (also designated Muscle embryonic myosin antibody) suitable for the detection of the MYH protein of mouse, rat and human origin. MYH Antibody (B-5) is available as both the non-conjugated anti-MYH antibody form, as well as multiple conjugated forms of anti-MYH antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Myosin is a highly conserved, ubiquitously expressed protein that interacts with Actin to generate the force for cellular movements. Conventional Myosins are hexameric proteins consisting of two heavy chain subunits, a pair of non-phosphorylatable light chain subunits and a pair of phosphorylatable light chain subunits. Three general classes of Myosin have been cloned: smooth muscle Myosins, striated muscle Myosins and non-muscle Myosins. Contractile activity in smooth muscle is regulated by the calcium/calmodulin-dependent phosphorylation of Myosin light chain (MLC) by Myosin light chain kinase. Myosin heavy chains, which are encoded by the MYH gene family, contain Actin-activated ATPase activity which generates the motor function of Myosin. Myosin heavy chains were initially isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Various isoforms of myosin heavy chains are differentially expressed depending on the functional activity of the muscle.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
To what part of MUTYH (MYH) does the antibody bind to? N-terminus, C-Terminus, or to the middle of the protein?
Preguntado por: cavasqu1
Thank you for your question. MYH Antibody (B-5): sc-376157 was raised against amino acids 1641-1940 of myosin heavy chain 3 of human origin, which corresponds to C-terminus of the protein.
Respondida por: Tech Support Europe
Fecha de publicación: 2020-07-29
Do mouse MYH2, MYH6, MYH7 can be detected by MYH(B-5) in WB?
Preguntado por: SYH05
Thank you for your question. This MYH antibody, sc-376157, will for sure detect MYH3, but has not been epitope mapped so we can not guarantee that it can pick up MYH2, MYH7 or MYH6. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Respondida por: Tech Service
Fecha de publicación: 2017-10-19
What is the recommended secondary reagent to be used with this mouse monoclonal primary?
Preguntado por: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here:
Rated 4 de
Erick G de
GoodMeets what was promised for WB, recognizes MYH in rat ventricular extract
Fecha de publicación: 2019-09-03
Rated 5 de
Great Technical Service and Great ProductI purchased this product one month ago and I must admit, I did have some bumps in the road when it came to receipt of the product and full understanding of the different variations. However, those bumps were easily smoothed out with a quick call to customer service and technical service helped me understand the product entirely. I'm very happy I ordered my antibodies from Santa Cruz Bio Technology.
Fecha de publicación: 2017-07-07
Rated 2 de
Weak for immunofluorescenceI used this antibody in thick sections (200 um) of breast xenografts. The staining is not very specific and weak.
Fecha de publicación: 2017-06-26
Rated 5 de
Very good in WB in C2C12 cellsCompare to MYH7 (A4.840), MYH （B5）shows strong signal in C2C12 differentiation for 7 days. It is a good antibody for WB in C2C12.
Fecha de publicación: 2017-05-31
Rated 5 de
Nice for WBThe MYH(B-5) mouse monoclonal antibody is very nice for Western blotting!
Fecha de publicación: 2017-05-24
Rated 5 de
Very good antibody for WBVery good antibody for WB and excellent immunohistochemistry, I suggest dilution this antibody at 1:200.
Fecha de publicación: 2017-02-09
Rated 5 de
superior replacementMYH Antibody (B-5) is raised against the same recombinant protein immunogen used to develop the rabbit polyclonal sc-20641 (H-300) antibody. This new monoclonal antibody is a superior replacement with greater specificity and no lot-to-lot variation.
Fecha de publicación: 2017-01-07
Rated 5 de
Excellent results in Western Blot with humanExcellent results in Western Blot with human, mouse and rat samples -SCBT QC
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