LHX5 CRISPR/Cas9 KO Plasmid (m): sc-421428
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- LHX5 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the LHX5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: LHX5 Antibody (KP-02): sc-130469
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
LHX5 CRISPR/Cas9 KO Plasmid (m) | sc-421428 | 20 µg | $397.00 |
Overview
Lhx5 encodes the LIM homeobox transcription factor LHX5, a nuclear DNA-binding regulator required for patterning and differentiation of the developing central nervous system. In mouse, LHX5 contributes to regional identity and neuronal lineage specification through LIM-domain–dependent transcriptional programs that intersect with broader homeobox networks controlling neurogenesis and axon guidance. Altered LHX5 activity is associated with defects in forebrain and hippocampal development and consequent neurodevelopmental phenotypes, making it a relevant target for mechanistic studies of neuronal circuit formation. As a transcriptional regulator, LHX5 influences downstream gene expression cascades that shape cell fate decisions and maturation states in neural progenitors and differentiating neurons.
LHX5 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Lhx5 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Lhx5 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Lhx5 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish LHX5 protein expression.
This CRISPR knockout system enables efficient generation of Lhx5-deficient cell models for investigation of LHX5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Lhx5 exon(s) critical for LHX5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Lhx5 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by LHX5 CRISPR/Cas9 KO Plasmid (m) and LHX5 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Lhx5 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by LHX5 HDR Plasmid (m) and LHX5 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Lhx5 homology arms to support homology-directed repair at defined Lhx5 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.