HNF-3 beta Anticuerpo (H-4) recomendado para detectarHNF-3β de mouse, rat y human origen, mediante WB, IP, IF y ELISA

HNF-3β Anticuerpo (H-4): sc-374376

HNF-3β Anticuerpo (H-4) clasificado 4.5 de 5 por 10.
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Ficha técnica

    • HNF-3β Anticuerpo (H-4) es un monoclonal de ratón IgG1 κ, ver las 31 publicaciones, proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 431-459 en el C-terminus de HNF-3β de origen mouse
    • HNF-3 beta Anticuerpo (H-4) es recomendado para detectar HNF-3β de mouse, rat y human origen, mediante WB, IP, IF y ELISA; también reactivo con otras especies, incluyendo anyd equine, canine, bovine and porcine
    • HNF-3 beta Anticuerpo (H-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • TransCruz reactivo para Gel Supershift y ChIP (sc-374376 X, 200 µg/0.1 ml)
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de HNF-3β (H-4): sc-374376.
    • m-IgGκ BP-HRP is the preferred secondary detection reagent for HNF-3β Antibody (H-4) for WB applications. This reagent is now offered in a bundle with HNF-3β Antibody (H-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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HNF-3β抗体(H-4): sc-374376の無料試供品をいただきたいのですが,可能でしょうか?ワークすれば購入の予定です.

Preguntado por: Anónimo
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Respondida por: Technical Support 15
Fecha de publicación: 2019-06-05

Hi, I plan on using this antibody for immunohistochemistry on floating brain tissue, would it be possible to get a sample of the antibody to test before we buy it? Thanks.

Preguntado por: Bondarenko
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Respondida por: Technical Support
Fecha de publicación: 2018-12-14

What application is the blocking peptide sc-374376 P appropriate for?

Preguntado por: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Very good antibody Works as we expected. Tested by immunofluorescence. Great
Fecha de publicación: 2018-12-18
Rated 5 de 5 por de Nuclear localization in IF The sample of the antibody worked quite well for IF in a dilution of 1:200
Fecha de publicación: 2018-01-24
Rated 3 de 5 por de Works but not specific We bought this antibody to detect FOXA2 in prostate cancer cell lines. Based on published literature, FOXA2 protein is expressed in PC-3 cells but not in LNCaP cells. As the figure showed, the antibody works but can pick up non-specific bands in LNCaP cells.
Fecha de publicación: 2017-12-15
Rated 4 de 5 por de ICC on iPSC DA neuronal culture I tested the antibody for an ICC on iPSC DA neuronal culture, fixed 20min in paraformaldehyde 4%. Dilution: 1/100. It worked but there is a little bit of noise.
Fecha de publicación: 2017-10-31
Rated 4 de 5 por de Immunohistochemistry free floating I tested the antibody for an immunohistochemistry on mouse embryo spinal cord at E12.5, fixed 1h in paraformaldehyde 4%. Dilution: 1/100, Permeabilization solution: PBS 1X- Triton 0,2% - Goat serum 10%. It worked but there is a little bit of noise.
Fecha de publicación: 2017-10-27
Rated 4 de 5 por de gute Western Blotergebnisse WB mit Zelllysaten, Verdünnung 1:200, gute Signale mit ein wenig Backgroundsignal
Fecha de publicación: 2017-10-18
Rated 5 de 5 por de HNF-3beta/FOXA2 in neonatal rat brain Clear immunoreactivity in neonatal (PN2) rat brain, and absence of immunoreactivity in adult rat brain. Note only specific brain regions were examined. Used at 1:50, signal may be stronger with different dilutions/conditions.
Fecha de publicación: 2017-08-22
Rated 5 de 5 por de great features WB on mice Liver sample,diluted with 1:200,which shows good quality and got bands very clearly.
Fecha de publicación: 2017-04-30
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