HNF-3 beta Anticuerpo (B-2) recomendado para detectarHNF-3β de mouse, rat y human origen, mediante WB, IP, IF y ELISA

HNF-3β Anticuerpo (B-2): sc-271103

HNF-3β Anticuerpo (B-2) clasificado 4.8 de 5 por 4.
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Ficha técnica

    • HNF-3β Anticuerpo (B-2) es un monoclonal de ratón IgG1 κ, ver las 4 publicaciones, proporcionado como 200 µg/ml
    • raised against amino acids 261-370 mapping within an internal region of HNF-3β of human origin
    • recomendado para detectarHNF-3β de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • TransCruz reactivo, para ChIP (sc-271103 X, 200 µg/0.1 ml)
    • Ver HNF-3β (H-4): sc-374376 para HNF-3β anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for HNF-3β Antibody (B-2). This work is in progress.
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What is the recommended fixation method for immunofluorescence staining with HNF-3β (B-2): sc-271103 monoclonal antibody?

Preguntado por: AbPolly
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Great Antibody for IF This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Beautiful IF staining.
Fecha de publicación: 2017-01-23
Rated 4 de 5 por de Our lab tried this antibody in IF and got Our lab tried this antibody in IF and got good staining pattern.
Fecha de publicación: 2015-09-08
Rated 5 de 5 por de Strong nuclear localization seen in IF with Strong nuclear localization seen in IF with HepG2 cells -SCBT QC
Fecha de publicación: 2015-02-11
Rated 5 de 5 por de Good results in WB with Hep G2 and A549 nuclear Good results in WB with Hep G2 and A549 nuclear extracts. -SCBT QC
Fecha de publicación: 2014-02-15
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