gp91-phox Anticuerpo (54.1) recomendado para detectargp91-phox de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)

gp91-phox Anticuerpo (54.1): sc-130543

gp91-phox Anticuerpo (54.1) clasificado 4.9 de 5 por 20.
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Ficha técnica

    • Anti-gp91-phox Anticuerpo 54.1 es un monoclonal de ratón IgG1 κ gp91-phox Anticuerpo, ver las 76 publicaciones, proporcionado como 200 µg/ml
    • raised against gp91-phox of human origin, with epitope mapping to amino acids 383-390
    • gp91-phox Anticuerpo (54.1) es recomendado para detectar gp91-phox de mouse, rat y human origen, mediante WB, IP, IF y IHC(P); también recomendado para detectar ERp57, Mox1, Nox3 and Nox4
    • gp91-phox Anticuerpo (54.1) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de gp91-phox (54.1): sc-130543.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for gp91-phox Antibody (54.1) for WB and IHC(P) applications. This reagent is now offered in a bundle with gp91-phox Antibody (54.1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Can I use this antibody conjugated with Phycoerythrin for IHC or IF? If yes, under which channel can I image the stained cells? Is there a protocol for IHC or IF for this antibody? Thank you

Preguntado por: Keertana
Thank you for your question. Anti-gp91-phox Antibody (54.1) and its conjugates are recommended for detection of gp91-phox of mouse, rat and human origin by WB, IP, IF and IHC(P). They are also recommended for detection of ERp57, Mox1, Nox3 and Nox4. You can find our recommended protocols for IHC and IF experiments under this link: https://www.scbt.com/resources/protocols PE absorption peaks in the visible light spectrum are measured at 495 and 545/566 nm.
Respondida por: Technical Support Europe
Fecha de publicación: 2020-10-12

Is it possible for me to have a free trial sample for the above antibody? Thank you!

Preguntado por: Renato
Thank you very much for your inquiry. Please send us an email to [email protected] with your complete delivery address, we will contact you back as soon as possible.
Respondida por: Technical Support Europe
Fecha de publicación: 2017-12-05

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Preguntado por: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-130543 PE) or fluoroscein (sc-130543 FITC), Alexa Fluor® 488 (sc-130543 AF488) or Alexa Fluor® 647 (sc-130543 AF647). Please contact Technical Service if you have further questions concerning this product.
Respondida por: Tech Service 9
Fecha de publicación: 2017-01-04
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Rated 5 de 5 por de good antibody the antibody works very well in IHC. No problem with background.
Fecha de publicación: 2020-12-01
Rated 5 de 5 por de Very good for Western Blotting Works great for western blotting (mouse heart lysate), can see clear bands at about 60 kDa.
Fecha de publicación: 2019-07-05
Rated 5 de 5 por de worked It works great - I 'd recommend this Nox2 antibody.
Fecha de publicación: 2018-03-16
Rated 5 de 5 por de Great IB antibody This antibody works great with mouse cells. We also included Nox2-KO cells as a genetic control.
Fecha de publicación: 2017-10-08
Rated 4 de 5 por de very good The strips are very clear and there are not many bands.
Fecha de publicación: 2017-08-27
Rated 5 de 5 por de IF worked in murine DC 2.4 cells It worked great with murine DC 2.4 cells. I did not try to optimize the concentration, but it worked with 1:50 as they recommended.
Fecha de publicación: 2017-07-08
Rated 5 de 5 por de This antibody work well for WB This antibody can also detect the samples from the pig ,it can replace the sc-5826 .
Fecha de publicación: 2017-06-23
Rated 5 de 5 por de Awesome in WB Great signal in WB. Detects a single band in HeLa lysate at a 1:1000 dilution.
Fecha de publicación: 2017-06-06
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