Anti-DPM1 Anticuerpo A-5 es un monoclonal de ratón IgG1 κ DPM1 Anticuerpo, ver las 1 publicaciones, proporcionado como 200 µg/ml
específico para un epítopo localizado entre los amino ácidos 78-102 de la región interna de DPM1 de origen human
DPM1 Anticuerpo (A-5) es recomendado para detectar DPM1 de mouse, rat y human origen, mediante WB, IP, IF y ELISA
DPM1 Anticuerpo (A-5) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de DPM1 (A-5): sc-515721.
m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for DPM1 Antibody (A-5) for WB applications. These reagents are now offered in bundles with DPM1 Antibody (A-5) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
DPM1 Antibody (A-5) is a high quality monoclonal DPM1 antibody (also designated DPM1 antibody) suitable for the detection of the DPM1 protein of mouse, rat and human origin. DPM1 Antibody (A-5) is available as both the non-conjugated anti-DPM1 antibody form, as well as multiple conjugated forms of anti-DPM1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Biosynthesis of glycosylphosphatidylinositol and N-glycan pre-cursor is dependent upon a mannosyl donor, dolichol phosphate-mannose (DPM). DPM synthase, a transmembrane protein, is associated with membranes of the rough endoplasmic reticulum and catalyzes mannosyl transfer from GDP-mannose hydrophobic long-chain acceptor dolichyl-phosphate (3-4). DPM synthase in various organisms are grouped into two types. One type is a single-component enzyme, represented by Saccharomyces cerevisiae, and the other is a multicomponent enzyme which is represented by human DPM synthase and consists of three subunits: DPM1, DPM2 and DPM3. DPM1 is not sufficient for DPM synthesis, which requires the 84 amino acid DPM2 protein for localization to the ER and stable expression of DPM1. The third subunit, DPM3, comprises 92 amino acids, and it is associated with DPM1 via its C-terminal domain and with DPM2 via its N-terminal region. The stability of DPM1 is directly dependent upon DPM3, which is stablized by DPM2. DPM synthase activity is associated with an ER phosphoprotein. In addition, a mitochondrial DPM synthase exists, which is located on the cytosolic face of the outer membrane of mitochondria.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
How much sample do you recommend loading on the gel for Western blot detection using DPM1 (A-5): sc-515721?
Preguntado por: jenni
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference:
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