cyclin D3 Anticuerpo (DCS-22) recomendado para detectarcyclin D3 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

cyclin D3 Anticuerpo (DCS-22): sc-56307

cyclin D3 Anticuerpo (DCS-22) clasificado 4.8 de 5 por 4.
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Ficha técnica

    • cyclin D3 Anticuerpo (DCS-22) es un monoclonal de ratón IgG1 κ, ver las 8 publicaciones, proporcionado como 200 µg/ml
    • producido contra la proteína completa cyclin D3 de origen human
    • cyclin D3 Anticuerpo (DCS-22) es recomendado para detectar cyclin D3 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • cyclin D3 Anticuerpo (DCS-22) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de cyclin D3 (DCS-22): sc-56307.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for cyclin D3 Antibody (DCS-22) for WB and IHC(P) applications. These reagents are now offered in bundles with cyclin D3 Antibody (DCS-22) (see ordering information below).
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I am using cyclin D3 (DCS-22): sc-56307 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Specific Western detection in rat INS-1 cells 0 ug loads of rat insulinoma (INS-1) lysates were probed overnight and returned a strong, specific band of ~38 kD. The attached picture is a triplicate set (identical lanes)
Fecha de publicación: 2019-05-22
Rated 4 de 5 por de a relative good antibody for WB This antibody generates a signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract), although the signal intensity is not strong. I would recommend other products (e.g. sc-453 or sc--6283) for WB
Fecha de publicación: 2017-09-18
Rated 5 de 5 por de Clean blot with single strong band in C6 Clean blot with single strong band in C6, K-562, PMA treated K-562 and Jurkat whole cell lysates. -SCBT QC
Fecha de publicación: 2015-03-26
Rated 5 de 5 por de This product works great for IHC paraffin This product works great for IHC paraffin-embedded human pancreas tissue. -SCBT QC
Fecha de publicación: 2014-06-19
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