Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CRY1 Double Nickase Plasmid (h): sc-400946-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CRY1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CRY1 Double Nickase Plasmid (h) and CRY1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CRY1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CRY1 Antibody (H-12): sc-393466
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRY1 Double Nickase Plasmid (h) | sc-400946-NIC | 20 µg | $410.00 |
CRY1 (cryptochrome circadian regulator 1) encodes a core component of the mammalian circadian clock that functions within the transcriptional–translational feedback loop to repress CLOCK–BMAL1-driven gene expression. By integrating with PER proteins and associated regulatory complexes, CRY1 helps coordinate rhythmic control of metabolism, cell cycle timing, DNA damage responses, and endocrine signaling across tissues. Altered CRY1 activity or expression has been linked to circadian rhythm disruption and downstream effects on sleep–wake regulation and metabolic homeostasis, and it is frequently studied in the context of clock-controlled transcriptional networks. These properties make CRY1 a useful node for investigating how temporal regulation shapes cellular physiology and stress adaptation.
CRY1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CRY1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CRY1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CRY1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CRY1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.