CENP-B Anticuerpo (C-10) es un monoclonal de ratón IgG2a κ, ver las 3 publicaciones, proporcionado como 200 µg/ml
producido contra los amino ácidos 535-599 localizados en el C-terminus de origen CENP-B of human
CENP-B Anticuerpo (C-10) es recomendado para detectar CENP-B de mouse, rat y human origen, mediante WB, IP, IF y ELISA
CENP-B Anticuerpo (C-10) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
Contacte con nosotros para recibir GRATIS 10 µg de muestra de CENP-B (C-10): sc-376392.
m-IgGκ BP-HRP is the preferred secondary detection reagent for CENP-B Antibody (C-10) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (C-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. Both centromere proteins CENP-B and CENP-H are contained in the centromeric heterochromatin between kinetochores, and are involved in maintaining sister chromatid cohesion. The highly dispersed CENP-B promotes and maintains the joining of DNA satellites in the centromere. CENP-B targets centromeric a-DNA and protects it from digestion by nucleases as well as preventing DNAse or restriction enzyme digestion from affecting the morphology of centromeres. CENP-H contains a coiled-coil structure and a nuclear localization signal. CENP-H is specifically and constitutively localized to kinetochores and plays a role in the organization and function of kinetochores throughout the cell cycle.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What does it mean if my test results are 149 for CENP A and 186 for CENP B?
Preguntado por: Jennylee
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Respondida por: Blake J
Fecha de publicación: 2021-08-25
What is the recommended fixation method for immunofluorescence staining with CENP-B (C-10): sc-376392 monoclonal antibody?
Preguntado por: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
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