Cdc7 Anticuerpo (SPM171) recomendado para detectar Cdc7 de human origen, mediante WB, IP, IF y IHC(P)

Cdc7 Anticuerpo (SPM171): sc-56275

Cdc7 Anticuerpo (SPM171) clasificado 4.6 de 5 por 7.
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Ficha técnica

    • Anti-Cdc7 Anticuerpo SPM171 es un monoclonal de ratón IgG1 κ Cdc7 Anticuerpo, ver las 6 publicaciones, proporcionado como 200 µg/ml
    • producido contra la proteína completa Cdc7 de origen human
    • recomendado para detectar Cdc7 de human origen, mediante WB, IP, IF y IHC(P)
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cdc7 (SPM171): sc-56275.
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc7 Antibody (SPM171) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc7 Antibody (SPM171) (see ordering information below).
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What is the recommended starting dilution for Western blot for sc-56275: Cdc7 (SPM171) monoclonal antibody?

Preguntado por: Germaine
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Respondida por: Technical Support
Fecha de publicación: 2017-03-22

How much is the volume of sc-56275? How many staining of specimen with moderate size is possible? Dilution has been mentioned in datasheet.(200 ug / ml)

Preguntado por: jaafariz
Thank you very much for your inquiry. The Cdc7 Antibody (SPM171): sc-56275 is provided at 200 µg in 1 ml buffer. The volume you will receive is 1 ml. We would recommend to use a starting dilution of 1:50 for IHC(P). The number of slides that you can stain with one vial depends on the volume you are using for incubating each slide. Please find further information in our protocols under the following link: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support Europe
Fecha de publicación: 2017-02-28
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Rated 5 de 5 por de Clean bands I requested a sample of this antibody and tested it four cell lines (DLD1, HeLa, RPE1 and U2OS) in different fractions (Whole cell, soluble and chromatin) and it detects a band of approximately 60 kDa.
Fecha de publicación: 2019-07-03
Rated 5 de 5 por de Good antibody Strong antibody for WB (1:100) dilution) tested in HCT116 whole cell lysates.
Fecha de publicación: 2018-02-19
Rated 5 de 5 por de Great antibody for western blot The batch of antibody I have worked well on BJAB, HeLa, Jurkat and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 250 times into 5% BSA, TBST and incubated over-night at 4°C.
Fecha de publicación: 2018-01-04
Rated 4 de 5 por de Good for WB Very strong and specific band for western blot in human cell samples
Fecha de publicación: 2017-10-12
Rated 4 de 5 por de Performs well in non Performs well in non-transfected 293T, human Cdc7 transfected 293T and HeLa whole cell lysates by WB. -SCBT QC
Fecha de publicación: 2015-02-22
Rated 5 de 5 por de Good specificity and single strength for Good specificity and single strength for western blot analysis of Cdc7 expression in non-transfected and human Cdc7 transfected 293T whole cell lysates. - SCBT QC
Fecha de publicación: 2015-02-05
Rated 4 de 5 por de Strong signal with a bit of background for Strong signal with a bit of background for WB in HeLa, ECV304 and Y79 whole cell lysates. - SCBT QC
Fecha de publicación: 2014-05-09
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