Cdc45 Anticuerpo (G-12) recomendado para detectarCdc45 de mouse, rat y human origen, mediante WB, IP, IF y ELISA

Cdc45 Anticuerpo (G-12): sc-55569

Cdc45 Anticuerpo (G-12) clasificado 4.4 de 5 por 8.
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Ficha técnica

    • Anti-Cdc45 Anticuerpo G-12 es un monoclonal de ratón IgG3 κ Cdc45 Anticuerpo, ver las 20 publicaciones, proporcionado como 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of Cdc45 of human origin
    • Cdc45 Anticuerpo (G-12) es recomendado para detectar Cdc45 de mouse, rat y human origen, mediante WB, IP, IF y ELISA
    • Cdc45 Anticuerpo (G-12) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cdc45 (G-12): sc-55569.
    • m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc45 Antibody (G-12) for WB applications. These reagents are now offered in bundles with Cdc45 Antibody (G-12) (see ordering information below).
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What is your recommended western blotting secondary antibody for use with Cdc45 (G-12): sc-55569?

Preguntado por: jenniferc
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Respondida por: Technical Support 4
Fecha de publicación: 2017-02-04
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Rated 4 de 5 por de Worked in WB Worked fine at 1:500 in 10% milk, though you can see some non-specific bands.
Fecha de publicación: 2017-08-30
Rated 1 de 5 por de Not useful for me It did not work in my hands. I saw a mix of bands, but none of them in the right place.
Fecha de publicación: 2017-08-21
Rated 5 de 5 por de Reliable results! Cdc45 monoclonal antibody (G-12) gives consistent and reliable results in WB and has been validated against Cdc45 transfectants, and for endogenous detection.
Fecha de publicación: 2017-01-19
Rated 5 de 5 por de Works well for IF applications Works well for IF applications, positive in methanol-fixed HeLa cells. -SCBT QC
Fecha de publicación: 2015-04-04
Rated 5 de 5 por de Publication in WB Publication in WB, FCM and IF. Good data obtained with human osteosarcoma cell line U2OS. -SCBT Publication Review
Fecha de publicación: 2015-04-02
Rated 5 de 5 por de WB: Detects endogenous Cdc45 in HeLa and WB: Detects endogenous Cdc45 in HeLa and Jurkat whole cell lysates. -SCBT QC
Fecha de publicación: 2015-02-22
Rated 5 de 5 por de WB: Single strong band in A WB: Single strong band in A-431 nuclear extract. -SCBT QC
Fecha de publicación: 2015-01-20
Rated 5 de 5 por de Worked with human transfected Cdc45 lysate by WB Worked with human transfected Cdc45 lysate by WB -SCBT QC
Fecha de publicación: 2014-07-30
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