Cdc37 Anticuerpo (E-4) recomendado para detectarCdc37 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

Cdc37 Anticuerpo (E-4): sc-13129

Cdc37 Anticuerpo (E-4) clasificado 4.9 de 5 por 11.
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Ficha técnica

    • Anti-Cdc37 Anticuerpo E-4 es un monoclonal de ratón IgG2b κ Cdc37 Anticuerpo, ver las 38 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 108-378 de Cdc37 de origen human
    • Cdc37 Anticuerpo (E-4) es recomendado para detectar Cdc37 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA
    • Cdc37 Anticuerpo (E-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • también disponibles conjugados a TRITC para IF, IHC(P) y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cdc37 (E-4): sc-13129.
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc37 Antibody (E-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc37 Antibody (E-4) (see ordering information below).
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Hi I would like to use this antibody (Cdc37 (E-4)) for immunoprecipitation. Has anyone tested it for this? How much antibody was used?

Preguntado por: Henriette
Thank you for your question. This antibody is recommended and warrantied for Immunoprecipitation use, and there are several publications in that application. Here is a link to our recommended protocol: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Respondida por: Tech Service
Fecha de publicación: 2017-08-28

I am using Cdc37 (E-4): sc-13129 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support 5
Fecha de publicación: 2017-01-25
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Rated 5 de 5 por de Excellent in WB Excellent in WB Dilution 1:1000 clear and specific band
Fecha de publicación: 2021-02-25
Rated 4 de 5 por de Great antibody This antibody works well for IP and also WB. Thank you, Santa Cruz.
Fecha de publicación: 2018-03-25
Rated 5 de 5 por de Good antibody This antibody worked very good for WB and IP. WB diluted 1:1000 in 293t cell lysate.
Fecha de publicación: 2017-09-28
Rated 5 de 5 por de very helpful This antibody is very helpful for analyisis cdc37 in HepG2 cell, I got clear and strong signal band at 50kda.This is the target band, no background by dilution 1;600.
Fecha de publicación: 2017-08-26
Rated 5 de 5 por de Pretty good Good specificity in WB in rat testis tissue, we usually use the dilution 1:300.
Fecha de publicación: 2017-03-18
Rated 5 de 5 por de versitile antibody Used this antibody for IHC(P), IF and WB in multiple samples, which produced nice and specific signal
Fecha de publicación: 2017-01-17
Rated 5 de 5 por de Good cytoplasmic staining of formalin fixed Good cytoplasmic staining of formalin fixed glandular cells. -SCBT Publication Review
Fecha de publicación: 2015-04-11
Rated 5 de 5 por de Strong WB band in Jurkat Strong WB band in Jurkat, CTLL-2 and KNRK whole cell lysates. -SCBT QC
Fecha de publicación: 2015-04-11
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