Cdc27 Anticuerpo (C-4) recomendado para detectarCdc27 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P), FCM y ELISA

Cdc27 Anticuerpo (C-4): sc-13154

Cdc27 Anticuerpo (C-4) clasificado 4.8 de 5 por 8.
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Ficha técnica

    • Cdc27 Anticuerpo (C-4) es un monoclonal de ratón IgG2a κ, ver las 14 publicaciones, proporcionado como 200 µg/ml
    • producido contra los amino ácidos 1-300 de Cdc27 de origen human
    • Cdc27 Anticuerpo (C-4) es recomendado para detectar Cdc27 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P), FCM y ELISA
    • Cdc27 Anticuerpo (C-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de Cdc27 (C-4): sc-13154.
    • m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdc27 Antibody (C-4) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdc27 Antibody (C-4) (see ordering information below).
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Can Cdc27 (C-4): sc-13154 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Preguntado por: DefinitelyNotMatt
Thank you for your question. Yes, Cdc27 (C-4): sc-13154 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-25
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Rated 5 de 5 por de good product This is a good product, pretty clean with only one non-specific band on U2OS cell line WB.
Fecha de publicación: 2017-12-26
Rated 5 de 5 por de Excellent Result I used Cdc27 for my WB. It gave me excellent result. I got very nice strong bands.
Fecha de publicación: 2017-09-29
Rated 5 de 5 por de satisfactorily We have data demonstrating that Cdc16 and Cdc27 are components of APC, and that ubiquitination of cyclin B leads to the degradation of the cyclin B / Cdk complex. It is very gratifying that the results of our experiments have been satisfactorily achieved
Fecha de publicación: 2017-02-02
Rated 5 de 5 por de Good results obtained by immunofluorescence Good results obtained by immunofluorescence when staining for mouse testis. -SCBT Publication Review
Fecha de publicación: 2015-04-10
Rated 4 de 5 por de This antibody was found to be suitable for This antibody was found to be suitable for use in flow cytometry applications using fixed and permeabolized K-562 cells. -SCBT QC
Fecha de publicación: 2015-04-08
Rated 5 de 5 por de Great antibody for human and rat immunoprecipitation, published using human and mouse cerebellar branules, HeLa and HEK293 cell lines. -SCBT Publication Review
Fecha de publicación: 2015-03-01
Rated 5 de 5 por de Strong band at correct molecular weight observed Strong band at correct molecular weight observed by Western Blot with K-562 whole cell lysate. -SCBT QC
Fecha de publicación: 2015-02-02
Rated 4 de 5 por de Good nuclear staining of surface epithelial Good nuclear staining of surface epithelial cells of formalin fixed paraffin embedded (PPFE) human esophagus tissue. -SCBT QC
Fecha de publicación: 2014-11-13
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