Cdc25A Anticuerpo (DCS-120) recomendado para detectarCdc25A de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)

Cdc25A Anticuerpo (DCS-120): sc-56264

Cdc25A Anticuerpo (DCS-120) clasificado 5.0 de 5 por 5.
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Ficha técnica

    • Cdc25A Anticuerpo (DCS-120) es un monoclonal de ratón IgG2a κ, ver las 8 publicaciones, proporcionado como 200 µg/ml
    • raised against purified recombinant Cdc25A
    • recomendado para detectarCdc25A de mouse, rat y human origen, mediante WB, IP, IF y IHC(P)
    • Ver Cdc25A (F-6): sc-7389 para Cdc25A anticuerpos conjugados, incluyendo AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 y 790.
    • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Cdc25A Antibody (DCS-120). This work is in progress.
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I am using Cdc25A (DCS-120): sc-56264 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Preguntado por: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de We really enjoyed Great IHC staining, it is better than the other, play a large role in the experiment.
Fecha de publicación: 2017-04-10
Rated 5 de 5 por de Excellent! This antibody works great in western blot analysis of Cdc25A expression in HeLa and K-562 whole cell lysates.
Fecha de publicación: 2017-02-01
Rated 5 de 5 por de Great cytoplasmic and membrane immunoperoxidase Great cytoplasmic and membrane immunoperoxidase staining observed on glandular cells of formalin fixed, paraffin embedded (FFPE) human upper stomach tissue. -SCBT QC
Fecha de publicación: 2015-03-28
Rated 5 de 5 por de Specificity shown for Cdc25A by testing by Specificity shown for Cdc25A by testing by Western Blot comparing 293T cells that have been transfected with the human Cdc25A protein to those that were not. No band observed for non-transfected cells, correct band observed for cells that were over-expressing the protein. -SCBT QC
Fecha de publicación: 2015-03-06
Rated 5 de 5 por de Very strong signal by Western Blot using Very strong signal by Western Blot using HeLa and K-562 whole cell lysates. -SCBT QC
Fecha de publicación: 2013-05-17
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